Pectic polysaccharide, homogalacturonan (monoclonal, clone LM20)
AS18 4193-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
DATA SHEET IN PDF| Data sheet | Product citations | Add review |
Product Information
Immunogen
Pectic polysaccharide, Homogalacturonan.
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgM
Format
Cell Culture Supernatant
Quantity
1ml
Storage
Store at +4°C (short term) and at -20°C (long term).
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses
Not reactive in
No confirmed exceptions from predicted reactivity known at the moment.
Application examples
Application examples
Immunfluorescence method described in:
An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.
Immunfluorescence method described in:
An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.
Additional information
Additional information
Contains 0.05% Sodium Azide
Has no known cross-reactivity with other polymers.
Binds to methyl esterified homogalacturonan.
Does not bind to un-esterified homogalacturonanl.
Has no known cross-reactivity with other polymers.
Binds to methyl esterified homogalacturonan.
Does not bind to un-esterified homogalacturonanl.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
Product citations
Selected references
Pan, Li, Liu, Qi et al. (2023) Multi-microscopy techniques combined with FT-IR spectroscopy reveals the histological and biochemical causes leading to fruit texture difference in oriental melon (Cucumis melo var. Makuwa Makino), Food Chemistry,Volume 402,ī2023,134229, ISSN 0308-8146, https://doi.org/10.1016/j.foodchem.2022.134229.(https://www.sciencedirect.com/science/article/pii/S0308814622021914)
Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.
Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.
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