Pectic polysaccharide, homogalacturonan (monoclonal, clone LM20)

AS18 4193-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
Pectic polysaccharide, homogalacturonan (monoclonal, clone LM20) in the group Antibodies Plant/Algal  / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4193-1ml)
Pectic polysaccharide, homogalacturonan (monoclonal, clone LM20)


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen Pectic polysaccharide, Homogalacturonan.
Host Rat
Clonality Monoclonal
Subclass/isotype IgM
Format Cell Culture Supernatant
Quantity 1ml
Storage Store at +4°C (short term) and at -20°C (long term).

Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution 1:10 (ELISA, IF)


Confirmed reactivity Higher plants, ferns and mosses
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.

Application examples

Application examples

Immunfluorescence method described in:
An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.

Additional information

Additional information Contains 0.05% Sodium Azide

Has no known cross-reactivity with other polymers.
Binds to methyl esterified homogalacturonan.
Does not bind to un-esterified homogalacturonanl.

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Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. 

Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

Product citations

Selected references Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.