Pectic polysaccharide, homogalacturonan (monoclonal, clone LM20)

Product no: AS18 4193-1ml
AS18 4193-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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Delivery:  7-10 business days
  • Product Info
  • Sub class: IgM
    Immunogen: Pectic polysaccharide, Homogalacturonan.
    Host: Rat
    Clonality: Monoclonal
    Format: Cell Culture Supernatant
    Quantity: 1ml
    Storage: Store at +4°C (short term) and at -20°C (long term).

    Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tubes.
    Tested applications: ELISA (ELISA), Immuniflourescence (IF)
    Recommended dilution: 1:10 (ELISA, IF)
  • Reactivity
  • Confirmed reactivity: Higher plants, ferns and mosses
    Not reactive in: No confirmed exceptions from predicted reactivity known at the moment.
  • Application Examples

  • Immunfluorescence method described in:
    An extended set of monoclonal antibodies to pectic homogalacturonan. Yves Verhertbruggen, Susan E. Marcus, Ash Haeger, José J. Ordaz-Ortiz, J. Paul Knox, Carbohydrate research 344 (2009) 1858-1862.

  • Additional Information
  • Additional information: Contains 0.05% Sodium Azide

    Has no known cross-reactivity with other polymers.
    Binds to methyl esterified homogalacturonan.
    Does not bind to un-esterified homogalacturonanl.
  • Background
  • Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins.  Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. 

    Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.
  • Product Citations
  • Selected references: Pan, Li, Liu, Qi et al. (2023) Multi-microscopy techniques combined with FT-IR spectroscopy reveals the histological and biochemical causes leading to fruit texture difference in oriental melon (Cucumis melo var. Makuwa Makino), Food Chemistry,Volume 402,2023,134229, ISSN 0308-8146, https://doi.org/10.1016/j.foodchem.2022.134229.(https://www.sciencedirect.com/science/article/pii/S0308814622021914)
    Verhertbruggen et al. (2009). An extended set of monoclonal antibodies to pectic homogalacturonan. Carbohydr Res. 2009 Sep 28;344(14):1858-62.doi: 10.1016/j.carres.2008.11.010.
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