Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16)

Name: Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16)
Brand: IKP
SKU: AS18 4201-1ml
Price: 246 EUR
Availability: InStock

AS18 4201-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16) in the group Antibodies Plant/Algal / Cell Wall / Dr. Knox collection, University of Leeds at Agrisera AB (Antibodies for research) (AS18 4201-1ml)

Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16)

Product Information

Immunogen Pectic polysaccharide, Rhamnogalacturonan

Host Rat

Clonality Monoclonal

Subclass/isotype IgM

Purity Cell culture supernatant.

Format Liquid

Quantity 1 ml

Storage Store at +4°C (short term) and at -20°C (long term).

Tested applications ELISA (ELISA), Immuniflourescence (IF)

Recommended dilution 1:10 (ELISA, IF)

Reactivity

Confirmed reactivity Higher plants, ferns and mosses

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Immunofluorescent visualization of rhamnogalacturonan in Nicotiana tabacum stem.
Applied immunolocalization method was as described in Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.

Additional information

Additional information Contains 0.05% Sodium Azide.

Reacts with polysaccharide, rhamnogalacturonan-I (RG-I) The binding could be sensitive to galactosidase action and the epitope could involve galactosyl residue(s) on the rhamnogalacturonan backbones.

Recognizes a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues.

Background

Background The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.

Product citations

Selected references Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.

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immunogen:	Pectic polysaccharide, Rhamnogalacturonan
Sub class:	IgM
Host:	Rat
Clonality:	Monoclonal
Purity:	Cell culture supernatant.
Format:	Liquid
Quantity:	1 ml
storage:	Store at +4°C (short term) and at -20°C (long term).
tested applications:	ELISA (ELISA), Immuniflourescence (IF)
recommended dilution:	1:10 (ELISA, IF)
Confirmed reactivity:	Higher plants, ferns and mosses
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Immunofluorescent visualization of rhamnogalacturonan in Nicotiana tabacum stem. Applied immunolocalization method was as described in Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
additional information:	Contains 0.05% Sodium Azide. Reacts with polysaccharide, rhamnogalacturonan-I (RG-I) The binding could be sensitive to galactosidase action and the epitope could involve galactosyl residue(s) on the rhamnogalacturonan backbones. Recognizes a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues.
background:	The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.
All references:	Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.