Pectic polysaccharide, Rhamnogalacturonan (monoclonal, clone LM16)
AS18 4201-1ml | Clonality: Monoclonal | Host: Rat | Reactivity: Higher plants, ferns and mosses
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom

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Product Information
Immunogen
Pectic polysaccharide, Rhamnogalacturonan
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgM
Purity
Cell culture supernatant.
Format
Liquid
Quantity
1 ml
Storage
Store at +4°C (short term) and at -20°C (long term).
Tested applications
ELISA (ELISA), Immuniflourescence (IF)
Recommended dilution
1:10 (ELISA, IF)
Reactivity
Confirmed reactivity
Higher plants, ferns and mosses
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples

Immunofluorescent visualization of rhamnogalacturonan in Nicotiana tabacum stem.
Applied immunolocalization method was as described in Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.

Immunofluorescent visualization of rhamnogalacturonan in Nicotiana tabacum stem.
Applied immunolocalization method was as described in Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
Additional information
Additional information
Contains 0.05% Sodium Azide.
Reacts with polysaccharide, rhamnogalacturonan-I (RG-I) The binding could be sensitive to galactosidase action and the epitope could involve galactosyl residue(s) on the rhamnogalacturonan backbones.
Reacts with polysaccharide, rhamnogalacturonan-I (RG-I) The binding could be sensitive to galactosidase action and the epitope could involve galactosyl residue(s) on the rhamnogalacturonan backbones.
Recognizes a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues.
Background
Background
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Pectins are the major polysaccharides that build pectic matrix of plant primary cell walls.
Product citations
Selected references
Verhertbruggen et al. (2009). Developmental complexity of arabinan polysaccharides and their processing in plant cell walls. Plant J. 2009 Aug;59(3):413-25.doi: 0.1111/j.1365-313X.2009.03876.x.
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