PELOTA | Protein pelota homolog
AS16 3984 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Solanum lycopersicum
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Recombinant PELOTA protein of Solanum lycopersicum, full length, Uniprot: K4BPD6
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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no confirmed exceptions from predicted reactivity are currently known
10 µg of total protein from Solanum lycopersicum overexpressing PELOTA GFP leaf extracted by SDS buffer and denatured at 100°C for 2-5 min. Proteins were separated on 10% SDS-PAGE minigel and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly trice and washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 sec with chemilominecent detection reagent.
Courtesy Dr. Moshe Reuveni, Plant Science Institute ARO, Volcani Center, Israel
Protein pelota homolog is involved in the mechanism for releasing non-functional ribosomes and degrading damaged mRNA. This protein belongs to the eukaryotic release factor 1 (ERF1) family, and the Pelota subfamily.