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PELOTA | Protein pelota homolog

AS16 3984   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Solanum lycopersicum

PELOTA | Protein pelota homolog in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS16 3984)

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Product Information

Immunogen

Recombinant PELOTA protein of Solanum lycopersicum, full length, Uniprot: K4BPD6

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity

50 µl

Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Expected | apparent MW

42 kDa

Reactivity

Confirmed reactivity Solanum lycopersicum
Predicted reactivity Arabidopsis lyrata, Arabidopsis thaliana, Beta vulgaris, Brassica napus, Brassica rapa subsp. pekinesis, Cajanus cajan, Capsella rubella, Cucumis sativus, Erythranthe guttata, Eutrema salsugineum, Eucalyptus grandis, Glycine max, Glycine soja, Gossypium raimondii, Jatropha curcas, Medicago truncatula, Nicotiana tabacum, Phaseolus angularis, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum sp., Spinacia oleracea, Theobroma cacao, Vigna angularis, Vitis vinifera.  

Species of your interest not listed? Contact us
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Western blot using anti-PELOTA antibodies

10 µg of total protein from Solanum lycopersicum overexpressing PELOTA GFP  leaf extracted by SDS buffer and denatured at 100°C for 2-5 min. Proteins were separated on 10% SDS-PAGE minigel and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly trice and washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 sec with chemilominecent detection reagent. 

Courtesy Dr. Moshe Reuveni, Plant Science Institute ARO, Volcani Center, Israel

Additional information

Antibody is recognizing recombinant PELOTA in fusion with GFP. Reactivity in endogenous material remains to be determined.

Related products

Background

Background

Protein pelota homolog is involved in the mechanism for releasing non-functional ribosomes and degrading damaged mRNA. This protein belongs to the eukaryotic release factor 1 (ERF1) family, and the Pelota subfamily.

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