PEPC | phosphoenolpyruvate carboxylase, DyLight® 650 conjugated (40 µg)

Product no: AS09 458-DL650
AS09 458-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri
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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4).

    For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.

    Format: Liquid in PBS pH 7,4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user
    Expected | apparent MW: 110 | 105 kDa
  • Reactivity
  • Confirmed reactivity: Ananas comosus, Arabidopsis thaliana, Synechocystis PCC 6803, Chromera velia, Hordeum vulgare, Jatropha curcas, Lupinus sp, , Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Saccharum spp, hybrid clone C91-301, Salsola laricifolia, Zea mays, Synechocystis sp, , Phaeodactylum tricornutum (strain CCAP 1055/1), Thalassiosira weissfloggi
    Predicted reactivity:
    Brassica napus, Cucumis sativus (PEPC1, PEPC2, PEPC3), Flaveria bidentis, Flaveria trinervia, Glycine max, Lupinus albus, Mammillaria thornberi, Manihot esculenta, Manihot obovata, Medicago sativa, Morinda citrifolia, Nannochloropsis gaditana CCMP526, Nopalea gaumeri, Opuntia macbridei, Pachycereus pringlei, Saccharum spp, Solanum tuberosum, Spinacia oleracea, Streptanthus tortuosus, Pachycereus hollianus, Pisum sativa, Phaseolus vulgaris, Populus sp.,Triticum aestivum, algae, diatoms: Thalassiosira pseudonana, other species: Salmonella sp., Schiedea hookeri, Shigella sp. Schiedea sarmentosa, Streptanthus farnsworthianus, Tacinga saxatilis,Yersinia sp. Vibrio sp., Quercus sp.

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known,
  • Additional Information
  • Additional information: DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
  • Background
  • Background: PEPC (phosphoenolpyruvate carboxylase), EC=4,1,1,31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate, Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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