Anti-RbcS | Ribulose-1,5-bisphosphate carboxylase/oxygenase
AS22 4825 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Data sheet
- Anti-RbcS | Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (Algal)
- Product Info
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Immunogen: KLH-conjugated peptide derived from Chlamydomonas reinhardtii RbcS, UniProt: P00873, P08475, Q31NB2 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 20 | 15 kDa - Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii, Synechococcus sp. PCC7002 Predicted reactivity: Arabidopsis thaliana
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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5 µg of total protein from C.reinhardtii,was denatured with Invitrogen LDS sample buffer (4X) at 70°C/5 min. Samples were separated on Invitrogen NuPage Bis-Tris 4-12% SDS-PAGE 60 min and blotted for 1 h to Invitrogen PVDF (pore size of 0.45 µm), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h/RT with TBS-T Blocking. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 10 min and 2 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG ALP conjugated, AS09 607 lot 2302) diluted to 1: 5 000 in TBS-T Blocking for 0,5h/RT with agitation. The blot was washed as above and developed with AS19 BCIP-NBT lot 03068231 for 30 seconds. Image was captured after 1h.
- Background
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Background: Rubisco catalyzes the rate-limiting step of carbon dioxide fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Benefits of using this antibody
Trial size limited to one per customer