RbcS | Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (Algal)

Product no: AS22 4825

AS22 4825 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Chlamydomonas reinhardtii RbcS, UniProt: P00873, P08475, Q31NB2
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 20 | 15 kDa
  • Reactivity
  • Confirmed reactivity: Chlamydomonas reinhardtii, Synechococcus sp. PCC7002
    Predicted reactivity: Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-algal RbcS antibodies

    5 µg of total protein from C.reinhardtii,was denatured with Invitrogen LDS sample buffer (4X) at 70°C/5 min.  Samples were separated on Invitrogen NuPage Bis-Tris 4-12% SDS-PAGE 60 min and blotted for 1 h to Invitrogen PVDF (pore size of  0.45  µm), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h/RT with TBS-T Blocking. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 10 min and 2 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG ALP conjugated, AS09 607 lot 2302) diluted to 1: 5 000 in TBS-T Blocking for 0,5h/RT with agitation. The blot was washed as above and developed with AS19 BCIP-NBT lot 03068231 for 30 seconds. Image was captured after 1h.

  • Background
  • Background: Rubisco catalyzes the rate-limiting step of carbon dioxide fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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