Name:
Phone:
E-mail:
Message


 


RbcL II | Rubisco large subunit, form II

AS15 2955 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  photosynthetic bacteria, archea, dinoflagellates


RbcL II | Rubisco large subunit, form II in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS15 2955)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide conserved in known RbcL form II protein sequences

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution

1:10 000 (WB) 

Expected | apparent MW

51.7 kDa

Reactivity

Confirmed reactivity Amphidinium carterae, Rhodobacter capsulatus, Rhodospirillum rubrum
Predicted reactivity Acidithiobacillus ferrooxidans, Dechloromonas aromatica, Gonyaulax polyedra, Leptothrix cholodnii, Magnetovibrio blakemorei, Rhodobacter sphaeroides, Thiobacillus denitrificans, Rhodopseudomonas palustris

and with a signle mismatch in one amino acid:
Gallionella capsiferriformans, Mariprofundus ferrooxydans, Thioalkalicoccus limnaeus, Methanomethylovorans hollandica, Methanococcoides burtonii, Methanosaeta concili, Methanolobus tindarius, Methanohalophilus mahii, and the dinoflagellate Symbiodinium sp. (ex Stylophora pistillata)
Species of your interest not listed? Contact us
Not reactive in Burkholderi, Cyanobium sp.

Application examples

Application examples

Application example


western blot using anti-RbcL form II antibodies

1.5 µg of total protein extract from Rhodobacter capsulatus (1); extracted with Agrisera Protein Extraction Buffer PEB (AS08 300); 0.5 pmol of recombinant RbcL I (2), 0.5 pmol of recombinant RbcL II (3) Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels,  LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent  or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS10 1489, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with chemiluminescent detection reagent of extreme femtogram sensitivity, according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.


Western blot using RbcL II antibody on dinoflagelates
Quantitative Immunoblot of Form II RbcL from Amphidinium carterae ( Dinoflagellata ). Algae were extracted from PC filters in Agrisera Protein Extraction Buffer (AS08 300) with 3 breakage cycles (60 seconds, 6.5 m/s) in a FastPrep-24 with D-matrix ceramic beads (MP Biomedicals). Total algal protein extract (2 μg, lanes 1-8) and a range of loads of recombinant form II RbcL standard (AS15 2955S, lanes 9-13, 37.5, 75, 150, 300, 450 fmol) was separated on a Bolt polyacrylamide gel (ThermoFisher) and bloSed onto PVDF. Following antibody incubations (primary antibody AS15 2955, 1:20000; secondary antibody AS09 602, 1:20 000), protein signal was developed using chemiluminescence detection reagent and
visualised using a VersaDoc Imager (BioRad).

Courtesy of Environmental Proteomics N.B, Canada

Additional information

Additional information The antibody does not detect Form I RbcL/cbbL.
Protein extraction from diatoms protocol can be found here.

Related products

Related products

AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)
AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)
AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated)

AS15 2955S | RbcL II | Rubisco form II positive control/quantitation standard

AS01 017  | anti-RbcL | Rubisco large subunit, form I, chicken antibody
AS01 017S | Rubisco protein standard for quantitative western blot or positive control

AS03 037PRE | Rubisco large subunit, pre-immune serum
AS09 409 | Rubisco quantitation kit
AS15 2994 | Rubisco ELISA quantitation kit 

AS07 218  | anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein

AS07 259
| anti-RbcS | Rubisco small subunit (SSU), rabbit antibody
AS07 222
| anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody

matching secondary antibody

Plant and algal protein extraction buffer

Background

Background

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms.

Form II Rubisco is present in many photosynthetic bacteria and archaea and in some photosynthetic dinoflagellates.

The large subunit (LSU) of form I Rubisco is encoded by the RbcL/cbbL gene, while form II LSU is encoded by cbbM.

Product citations

Selected references Bausch et al. (2019). Combined effects of simulated acidification and hypoxia on the harmful dinoflagellate Amphidinium carterae. Marine Biology, June 2019, 166:80.
Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.

Related products: RbcL II | Rubisco large subunit, form II

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
195 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS15 2955S  |  protein standard for quantitation and positive control
183 €
Buy 2 items of this product for 147.00 €/items
Buy 3 items of this product for 126.00 €/items