Pht1-1/2 | Root phosphate transporter isoform 1-1 and 1-2

Product no: AS08 321

AS08 321  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: PHT1-1 and 1-2 isoforms in Hordeum vulgare

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 µl
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
    Recommended dilution: 1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
    Expected | apparent MW: 56.9 kDa
  • Reactivity
  • Confirmed reactivity: Hordeum vulgare
    Predicted reactivity:

    Triticum aestivum

    Species of your interest not listed? Contact us
    Not reactive in:

    Arabidopsis thaliana

  • Additional Information
  • Additional information (application): Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation
  • Background
  • Background:

    Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).

  • Product Citations
  • Selected references: Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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