Pht1-1/2 | Root phosphate transporter isoform 1-1 and 1-2

AS08 321 | Clonality: Polyclonal | Host: Rabbit | Reactivity: PHT1-1 and 1-2 isoforms in Hordeum vulgare

Product Information

Immunogen

two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)

Expected | apparent MW 56.9 kDa

Reactivity

Confirmed reactivity Hordeum vulgare

Predicted reactivity

Triticum aestivum

Species of your interest not listed? Contact us

Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation

Background

Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).

Product citations

Selected references Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)

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All references:	Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
additional information (application):	Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation
background:	Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).
calculated \| apparent molecular mass [kDa]:	56.9 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Confirmed reactivity:	Hordeum vulgare
predicted reactivity:	Triticum aestivum Species of your interest not listed? Contact us
not reactive in:	Arabidopsis thaliana