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Pht1-1/2 | Root phosphate transporter isoform 1-1 and 1-2

AS08 321  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: PHT1-1 and 1-2 isoforms in Hordeum vulgare

Pht1-1/2 | Root phosphate transporter isoform 1-1 and 1-2 in the group Antibodies Plant/Algal  / Plant Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS08 321)



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Product Information

Immunogen

two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
Expected | apparent MW 56.9 kDa

Reactivity

Confirmed reactivity Hordeum vulgare
Predicted reactivity

Triticum aestivum


Species of your interest not listed? Contact us
Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation

Related products

Background

Background

Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).

Product citations

Selected references Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
immunogen:

two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH

Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 100 µl
Reconstitution: For reconstitution add 100 µl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
recommended dilution: 1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
Expected | apparent MW: 56.9 kDa
Confirmed reactivity: Hordeum vulgare
predicted reactivity:

Triticum aestivum


Species of your interest not listed? Contact us
not reactive in:

Arabidopsis thaliana

additional information (application): Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation
background:

Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).

All references: Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)

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