Anti-Phy | Phytochrome (clone Oat-25)

Product no: AS20 4500
AS20 4500 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avena sativa, Pisum sativum
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Sub class: IgG
    Immunogen: Phytochrome
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin Protein G purified, in PBS pH 7.4, 150 mM NaCl, 0.05 % of sodium azide.
    Format: Liquid
    Quantity: 100 µg
    Storage: Store at -20°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Competitive ELISA, Immunoprecipiation (IP), Western blot (WB)
    Recommended dilution: assay dependent
    Expected | apparent MW: 124 kDa
  • Reactivity
  • Confirmed reactivity: Avena sativa, Pisum sativum!!AIR8!!Avena sativa, Pisum sativum
    Predicted reactivity: graminae, fabaceae
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): Epitope recognized by this antibody is located at N-terminus of phytochrome, This domain undergoes conformational change when phytochrome is interconverted between the red and far-red absorbing forms
  • Background
  • Background: Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.
  • Product Citations
  • Selected references: Pavlovič et al. (2024). Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state. Physiol Plantarum, Volume176, Issue 1.
    Pratt et al. (1988). Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 267(2):723-35. doi: 10.1016/0003-9861(88)90081-1.
    Cordonnier et al. (1983). Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 158(4):369-76. doi: 10.1007/BF00397340.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.


AS07 220   |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
AS20 4496 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avena sativa, Pisum sativum
331 €
Add to cart

AS12 1867 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

319 €
Add to cart
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items