IKP

Phy | Phytochrome (clone Oat-25)

Name: Phy | Phytochrome (clone Oat-25)
Brand: IKP
SKU: AS20 4500
Price: 331 EUR
Availability: InStock

Product no: AS20 4500

AS20 4500 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avena sativa, Pisum sativum

331 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Sub class:	IgG
Immunogen:	Phytochrome
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin Protein G purified, in PBS pH 7.4, 150 mM NaCl, 0.05 % of sodium azide.
Format:	Liquid
Quantity:	100 �g
Storage:	Store at -20�C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	ELISA (ELISA), Competitive ELISA, Immunoprecipiation (IP), Western blot (WB)
Recommended dilution:	assay dependent
Expected \| apparent MW:	124 kDa

Reactivity

Confirmed reactivity:	Avena sativa, Pisum sativum
Predicted reactivity:	graminae, fabaceae Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Additional Information

Additional information (application):

Epitope recognized by this antibody is located at N-terminus of phytochrome, This domain undergoes conformational change when phytochrome is interconverted between the red and far-red absorbing forms

Background

Background:

Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.

Product Citations

Selected references:

Pratt et al. (1988). Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 267(2):723-35. doi: 10.1016/0003-9861(88)90081-1.
Cordonnier et al. (1983). Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 158(4):369-76. doi: 10.1007/BF00397340.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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