PhyA | Phytochrome A
AS07 220 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum

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Product Information
KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)
124 kDa
Reactivity
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Application examples

10 µg of total protein extracted with PEB (AS08 300) from etiolated seedlings of Arabidopsis thaliana (1) was separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 2h (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PhyA (AS07 220, 1:1000, 19.5h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity).

Reactant: Nicotiana tabacum (Common tobacco)
Application: Western Blotting
Pudmed ID: 28207924
Journal: J Exp Bot
Figure Number: 5B
Published Date: 2017-02-01
First Author: Prieto-Dapena, P., Almoguera, C., et al.
Impact Factor: 6.088
Open PublicationEffects of HSFA9 in seeds and seedlings before the first exposure to light. (A) Changes in transcript levels for COP1, PHYA, PHYB, and HY5 in DS10:A9 seeds, and converse trend as determined by RT-qPCR, in seeds from the loss-of-function DS10:A9-SRDX lines. The seeds were imbibed under darkness for 24 h. Two to three different DS10:A9 or two to four DS10:A9-SRDX pairs of homozygous transgenic/non-transgenic (NT) sibling lines were analysed in at least two experiments. Error bars denote the SEM. The asterisks denote that all fold-changes were statistically significant (the depicted ratios were different from 1; see Supplementary Table S3). (B) Enhanced accumulation of phyA and phyB antibody-reacting proteins under darkness in imbibed seeds and seedlings. Top: seeds imbibed for 24 h, results depicted for the indicated sibling pairs of NT/DS10:A9 and NT/DS10:A9-SRDX (A9-SRDX) lines. Bottom: seedling results illustrated for two different sibling pairs of NT and 35S:A9 lines. The putative phyA band disappears (as expected) after illumination for 6 h with white light (Light). Equal loading of total protein in samples was verified with Ponceau S staining (P).
Additional information
Background
Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.
Product citations
Schenk et al. (2021) Light-induced degradation of SPA2 via its N-terminal kinase domain is required for photomorphogenesis, Plant Physiology, 2021;, kiab156, https://doi.org/10.1093/plphys/kiab156
Menon et al. (2019). Arabidopsis FAR-RED ELONGATED HYPOCOTYL 1 and FHY1-LIKE are not required for phytochrome A signal transduction in the nucleus. Plant Communications. Available online 9 November 2019, 100007.
Agliassa et al. (2018). Geomagnetic field impacts on cryptochrome and phytochrome signaling. J Photochem Photobiol B. 2018 Aug;185:32-40. doi: 10.1016/j.jphotobiol.2018.05.027.
Zhang et al. (2018). Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13(5):e0198041. doi: 10.1371/journal.pone.0198041.
Pierto-Dapena et al. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. J Exp Bot. 2017 Feb 16. doi: 10.1093/jxb/erx020.
Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.
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