PhyA | Phytochrome A
AS07 220 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum
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KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)
10 µg of total protein extracted with PEB (AS08 300) from etiolated seedlings of Arabidopsis thaliana (1) was separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 2h (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PhyA (AS07 220, 1:1000, 19.5h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity).
Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.
Zhang et al. (2018). Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13(5):e0198041. doi: 10.1371/journal.pone.0198041. Pierto-Dapena et al. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. J Exp Bot. 2017 Feb 16. doi: 10.1093/jxb/erx020.
Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.