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Tag Antibody Set 

PhyA | Phytochrome A

AS07 220   |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum

PhyA | Phytochrome A in the group Antibodies for Plant/Algal  / Developmental Biology / Photomorphogenesis at Agrisera AB (Antibodies for research) (AS07 220)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis  thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

124 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Arachis hypogaea L., Nicotiana benthamiana, Nicotiana tabacum
Predicted reactivity Brassica rapa, Cardamine hirsuta, Lathyrus sativus, Fragaria ananassa, Glycine max, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples
Application example
Application example

10 µg of total protein extracted with PEB (AS08 300) from etiolated seedlings of Arabidopsis thaliana (1) was separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 2h (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PhyA (AS07 220, 1:1000, 19.5h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (300s, standard sensitivity).

Additional information

Additional information In vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.
Careful sample collection is adviced to assure the best results with this antibody.

Related products

Related products

AS06 198 | Anti-FT/TSF | flowering locus T and twin sister of FT, rabbit antibodies
AS18 4170 | Anti-PhyB | Phytochrome B, rabbit antibodies
AS16 3147 | Anti-SPA1 | supressor of PhyA-105, rabbit antibodies
AS12 2112 | Anti-PIF5 | phytochrome interacting factor 5, rabbit antibodies

Background

Background

Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.

Product citations

Selected references Agliassa et al. (2018). Geomagnetic field impacts on cryptochrome and phytochrome signaling. J Photochem Photobiol B. 2018 Aug;185:32-40. doi: 10.1016/j.jphotobiol.2018.05.027.
Zhang et al. (2018). Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13(5):e0198041. doi: 10.1371/journal.pone.0198041. Pierto-Dapena et al. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. J Exp Bot. 2017 Feb 16. doi: 10.1093/jxb/erx020.
Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.

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