IKP

Anti-Phy | Phytochrome (clone Oat-8)

Product no: AS20 4496
AS20 4496 | Clonality: Monoclonal | Host: Mouse | Reactivity: Avena sativa, Pisum sativum
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  • Product Info
  • Sub class: IgG2b
    Immunogen: Phytochrome
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin Protein G purified, in PBS pH 7.4, 150 mM NaCl, 0.05 % of sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at -20C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunoprecipiation (IP), Western blot (WB)
    Recommended dilution: assay dependent
    Expected | apparent MW: 124 kDa
  • Reactivity
  • Confirmed reactivity: Avena sativa, Pisum sativum
    Predicted reactivity: graminae, fabaceae
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: This antibody can also be used in competitive ELISA.

    Epitope recognized by this antibody is located ca. 70 kDa from N-terminus.
  • Background
  • Background: Phytochrome is a photomorphogenically active pigment that modulates plant growth and development with respect to incident light intensity and wavelength distribution. It exists in two forms: an inactive, red-absorbing form (Pr),4 and an active far-red-absorbing form (Pfr). When either absorbs light, it is photoconverted to the other. Phytochrome is a dimeric, water-soluble, relatively labile chromoprotein with similar, if not identical, monomers of about 124 kDa each. It is also a relatively low abundance protein, even under the best of conditions. Genetic manipulation of phytochrome expression in plants leads to plants requiring less light and able to divert more energy to the production of fruits and seeds. For its physicochemical characterization, it has therefore been difficult to utilize techniques that require large quantitites of highly purified protein. Consequently, indirect methods for elucidating its structure/function relationships are especially important. These could also be applicable to fabaceae and closely related families.
  • Product Citations
  • Selected references: Pratt et al. (1988). Mapping of antigenic domains on phytochrome from etiolated Avena sativa L. by immunoblot analysis of proteolytically derived peptides. Arch Biochem Biophys. 267(2):723-35. doi: 10.1016/0003-9861(88)90081-1.
    Cordonnier et al. (1983). Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta. 158(4):369-76. doi: 10.1007/BF00397340.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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