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AGO set
Tag Antibody Set 

CDJ1 | chloroplast DnaJ homolog 1

AS06 179  |  Clonality: Polyclonal  | Host: Rabbit  |  Reactivity:Chlamydomonas reinhardtii

CDJ1 | chloroplast DnaJ homolog 1 in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS06 179)

PRODUCT INFORMATION IN PDF

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355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1. 3000 (WB)
Expected | apparent MW

40 | 40 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity

Ostreococcus sp.

Not reactive in Higher plants

Application examples

Additional information

Western blot has been done on 10-50 ĩg load per well; immunoprecipitation has been done on 5-50 ĩl.

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Background

Background

CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

Product citations

Selected references Willmund et al (2008). The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C. Plant Physiol. 148: 2070-2082.

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