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AGO set
Tag Antibody Set 

Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1

AS08 345  |  Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Pisum sativum

Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1 in the group Antibodies for Plant/Algal  / Photosynthesis  / Chaperones/Transporters at Agrisera AB (Antibodies for research) (AS08 345)

PRODUCT INFORMATION IN PDF


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Product Information

Immunogen

psTOC75; Predicted POTRA Domain #1; Amino acids, 158-241; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL),Western blot (WB)
Recommended dilution 1 : 500 (Flow cyt). 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
Expected | apparent MW

88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Reactivity

Confirmed reactivity Pisum sativum, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus
Predicted reactivity Ricinus communis, Vitis vinifera, Populus trichocarpa
Not reactive in Physcomitrella patens

Application examples

Application examples

application example

580 ng of Chl of Pisum sativum plants (10 day old) (2, 4)  and  10 µg of combined envelopes of Pisum sativum 10 day-old (1,3)  were separated on 15% SDS-PAGE and blotted 2h to PVDF. PVDF was blocked 1h with 3% non-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 POTRA domain 1 antibodies AS08 345 (1:2000 and 1: 5000, 1h) and secondary donkey-anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 3% non fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with HRP substrate Peroxide solution & luminol detection reagent according to the manufacturers instructions (Millipore). Exposure time was 600 seconds.

Courtesy of Ashita Dave and Barry D. Bruce (UTK)

 

  western blot using anti-TOC75 POTRA domain 1 antibodies

Additional information

Antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction.

Related products

Related products

AS08 351 | Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

Plant protein extraction buffer

Secondary antibodies

Background

Background

OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane;  YaeT; outer membrane protein assembly complex, (TIGR03303);  FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Product citations

Selected references

to be added when available, antibody released February 2010