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AGO set
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Rubisco | 557 kDa hexadecamer

AS07 218  |  serum  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:  Plants and green algae

Rubisco | 557 kDa hexadecamer in the group Antibodies for Plant/Algal  / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS07 218)

PRODUCT INFORMATION IN PDF

Qty: 
332 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000), UniProt: P00875 and Q43832

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 10 000-1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard (WB). 1 : 500-1:1000 (IL)
Expected | apparent MW

53-55 | 53-55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Aucuba japonica, Chlamydomonas reinhardtii, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Physcomitrella patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Predicted reactivity Begonia sp., Daucus carota
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

0.5 µg of total leaf protein isolated with Agrisera Protein Eextraction Buffer (AS08 300) from Arabidopsis thaliana (1), Spinacia oleracea (2), Zea mays (3), Hordeum vulgare (4), Solanum tuberosum (5), Pisum sativum (6) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 45 min (30V) to nitrocellulose.  Filters where blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with AS07 218 (1:20 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder.  Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescence using a Fuji LAS-3000 CCD (300s, high sensitivity).

Additional information

RbcS subunit is not detected by this antibody

This product can be sold containing proclin if requested

Related products

Related products

AS03 037 | anti-RbcL | Rubisco large subunit, form I and form II (50 µl)
AS03 037A | anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified)

AS03 037-HRP| anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated)

AS15 2955
| anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibody
AS15 2955S | RbcL II | Rubisco form II positive control/quantitation standard

AS01 017  | anti-RbcL | Rubisco large subunit, form I, chicken antibody
AS01 017S | Rubisco protein standard for quantitative western blot or positive control

AS03 037PRE | Rubisco large subunit, pre-immune serum
AS09 409 | Rubisco quantitation kit
AS15 2994 | Rubisco ELISA quantitation kit 

AS07 259 | anti-RbcS | Rubisco small subunit (SSU), rabbit antibody
AS07 222
| anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibody

matching secondary antibody

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Product citations

Selected references Rohnke et al. (2018). RcaE-Dependent Regulation of Carboxysome Structural Proteins Has a Central Role in Environmental Determination of Carboxysome Morphology and Abundance in Fremyella diplosiphon. Mol Biol and Physiol. Vol. 3, Issue 1. DOI: 10.1128/mSphere.00617-17
Zhang et al. (2017). Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.
Wang et al. (2017). Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27(21):3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.
Wen and Zhang (2014). Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae). Photosynth Res. 2014 Sep 17. (immunolocalization)
Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.

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