Rubisco | 557 kDa hexadecamer

AS07 218 | Clonality: Polyclonal | Host: Rabbit | Reactivity: higher plants, lichens, algae

Product Information

Immunogen

purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000), UniProt: P00875 and Q43832

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 10 000-1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard (WB). 1 : 500-1:1000 (IL)

Expected | apparent MW

53-55 | 53-55 kDa

Reactivity

Confirmed reactivity Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrella patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays

Predicted reactivity higher plants, algae, Nannochloropsis sp.

Not reactive in Chlamydomonas reinhardtii (immulolocalization)

Application examples

Application examples Application example

0.5 µg of total leaf protein isolated with Agrisera Protein Eextraction Buffer (AS08 300) from Arabidopsis thaliana (1), Spinacia oleracea (2), Zea mays (3), Hordeum vulgare (4), Solanum tuberosum (5), Pisum sativum (6) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 45 min (30V) to nitrocellulose. Filters where blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with AS07 218 (1:20 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescence using a Fuji LAS-3000 CCD (300s, high sensitivity).

Additional information

RbcS subunit is not detected by this antibody

This product can be sold containing proclin if requested

Related products

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AS15 2955 | Anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodies
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Complete Collection of antibodies to Rubisco

Plant and algal protein extraction buffer

Background

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Product citations

Selected references Mihara et al. (2019). Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner. J Exp Bot. 2019 Dec 21. pii: erz561. doi: 10.1093/jxb/erz561.
Sedaghatmehr et al. (2019). A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. 2019 Mar;42(3):1054-1064. doi: 10.1111/pce.13426.
Rohnke et al. (2018). RcaE-Dependent Regulation of Carboxysome Structural Proteins Has a Central Role in Environmental Determination of Carboxysome Morphology and Abundance in Fremyella diplosiphon. Mol Biol and Physiol. Vol. 3, Issue 1. DOI: 10.1128/mSphere.00617-17
Zhang et al. (2017). Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.
Wang et al. (2017). Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27(21):3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.
Wen and Zhang (2014). Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae). Photosynth Res. 2014 Sep 17. (immunolocalization)
Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.