Rubisco | 557 kDa hexadecamer
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53-55 | 53-55 kDa
0.5 µg of total leaf protein isolated with Agrisera Protein Eextraction Buffer (AS08 300) from Arabidopsis thaliana (1), Spinacia oleracea (2), Zea mays (3), Hordeum vulgare (4), Solanum tuberosum (5), Pisum sativum (6) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 45 min (30V) to nitrocellulose. Filters where blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with AS07 218 (1:20 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescence using a Fuji LAS-3000 CCD (300s, high sensitivity).
RbcS subunit is not detected by this antibody
This product can be sold containing proclin if requested
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
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Rohnke et al. (2018). RcaE-Dependent Regulation of Carboxysome Structural Proteins Has a Central Role in Environmental Determination of Carboxysome Morphology and Abundance in Fremyella diplosiphon. Mol Biol and Physiol. Vol. 3, Issue 1. DOI: 10.1128/mSphere.00617-17
Zhang et al. (2017). Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.
Wang et al. (2017). Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27(21):3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.
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