HemC | Porphobilinogen-Deaminase (PBG)

AS08 375 | Clonality:Polyclonal | Host:Rabbit | Reactivity:Thermosynechoccus elongatus

HemC | Porphobilinogen-Deaminase (PBG) in the group Plant/Algal Antibodies / Photosynthesis  / Chlorophyll at Agrisera AB (Antibodies for research) (AS08 375)


355 €

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product information

PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.


overexpressed Thermosynechococcus elongatus HemC Q8DIE4

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS07 226 | anti-HemF

AS07 228 | anti-HemH | protoporphyrin ferrochelatase

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

34.7 kDa

Confirmed reactivity Thermosynechococcus elongatus
Predicted reactivity Cyanobacteria
Not reactive in

Synechococcus sp. PCC 6803

Additional information
Selected references

to be added when available

application example

0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon


wesern blot detection using anti-PBG (HemC) antibodies 

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