PLGG1 | Plastidal glycolate/glycerate translocator 1, (chloroplastic)

AS19 4293 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum

PLGG1 | Plastidal glycolate/glycerate translocator 1, (chloroplastic)

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana UniProt: Q9FVQ4

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 54 kDa

Reactivity

Confirmed reactivity Nicotiana tabacum

Predicted reactivity Arabidopsis thaliana, Noccaea caerulescens
Species of your interest not listed? Contact us

Not reactive in Vigna radiata

Application examples

Additional information

Experimental contitions:
5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl₂. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation.

According to South et. al (2019).

Background

Background PLGG1 (Plastidal glycolate/glycerate translocator) is a glycolate/glycerate transporter protein required for photorespiration

Alternative names of protein: AtLrgB, LrgB, PLGG

Product citations

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calculated \| apparent molecular mass [kDa]:	54 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana UniProt: Q9FVQ4
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl, of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Nicotiana tabacum
predicted reactivity:	Arabidopsis thaliana, Noccaea caerulescens Species of your interest not listed? Contact us
not reactive in:	Vigna radiata
background:	PLGG1 (Plastidal glycolate/glycerate translocator) is a glycolate/glycerate transporter protein required for photorespiration Alternative names of protein: AtLrgB, LrgB, PLGG
additional information (application):	Experimental contitions: 5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl₂. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation. According to South et. al (2019).