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Anti-PLGG1 | Plastidal glycolate/glycerate translocator 1, (chloroplastic)
AS19 4293 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana UniProt: Q9FVQ4 Host: Rabbit
Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 54 kDa - Reactivity
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Confirmed reactivity: Nicotiana tabacum Predicted reactivity: Arabidopsis thaliana, Noccaea caerulescens
Species of your interest not listed? Contact usNot reactive in: Solanum lycopersicum, Vigna radiata - Additional Information
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Additional information (application): Experimental contitions:
5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation.
According to South et. al (2019). - Background
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Background: PLGG1 (Plastidal glycolate/glycerate translocator) is a glycolate/glycerate transporter protein required for photorespiration
Alternative names of protein: AtLrgB, LrgB, PLGG - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS19 4295 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum
AS09 458 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, Ch. quinoa, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri
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