MS | Malate synthase, (glyoxysomal)
AS19 4295 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum
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5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation.
According to South et. al (2019).
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