MS | Malate synthase, (glyoxysomal)

Product no: AS19 4295

AS19 4295  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Nicotiana tabacum

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Cucurbita maxima UniProt: P24571
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 65 kDa
  • Reactivity
  • Confirmed reactivity: Nicotiana tabacum
    Predicted reactivity: Arabidopsis thaliana, Cajanus cajan, Cinnamomum micranthum f. kanehirae, Cucumis sativus, Cucurbita maxima, Fagus sylvatica, Glycine max, Jatropha curcas, Morus notabilis, Mucuna pruriens, Parasponia andersonii, Populus alba x tremula, Theobroma cacao, Trema orientale 
    Species of your interest not listed? Contact us
    Not reactive in: Sorghum bicolor
  • Additional Information
  • Additional information (application): Experimental contitions: 
    5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE  and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation. 

    According to South et. al (2019).
  • Background
  • Background: Malate synthase is involved in the synthesis of (S)-malate from isocitrate, where it catalyses the reaction: acetyl-CoA + glyoxylate + H(2)O = (S)-malate + CoA + H(+). This is part of the glyoxylate cycle, which is itself part of carbohydrate metabolism.
  • Product Citations
  • Selected references: South et. al (2019). Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field. Science 2019 Jan 4;363(6422), DOI: 10.1126/science.aat9077
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS19 4293   | Clonality: Polyclonal  |  Host: Rabbit | Reactivity: Nicotiana tabacum

302 €
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS09 458 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. comosus, A. thaliana, C. ciliaris, C. gayana, C. velia, Ch. quinoa, H. vulgare, J. curcas, K. prostrata, L. fusca, Lupinus sp. , M. maximus, M. crystallinum, N. tabacum, O. sativa, P. antidotale, P. coloratum, P. strobus, Saccharum spp. hybrid clone C91-301, S. lanata, S. laricifolia, S. bicolor, Synechocystis PCC 6803, Phaeodactylum tricornutum (strain CCAP 1055/1), T. weissfloggi, Z. mays, Z. muelleri

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Buy 2 items of this product for 226.00 €/items
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