showForm Anti-PPI | papain antibodies

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PPI | papain

AS09 545 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

PPI | papain  in the group Antibodies for Plant/Algal  / Food Proteins at Agrisera AB (Antibodies for research) (AS09 545)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

native papain isolated and purified from Carica papaya

Host Rabbit
Clonality Polyclonal
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

38.9 kDa

Reactivity

Confirmed reactivity Carica papaya
Predicted reactivity Carica papaya
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Related products

Related products

AS09 546 | anti-PPI | papain (biotinylated)

AS09 547 | anti-PPI | papain (affinity purified)

Plant protein extraction buffer

Secondary antibodies

Background

Background

Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.

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