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PPI | papain

AS09 545 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

PPI | papain  in the group Antibodies Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 545)
PPI | papain



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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

native papain isolated and purified from Carica papaya

Host Rabbit
Clonality Polyclonal
Purity Purified IgG in PBS.
Format Lyophilized
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW 38,9 kDa

Reactivity

Confirmed reactivity Carica papaya
Predicted reactivity Carica papaya
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Related products

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AS09 546 | Anti-PPI | papain (biotinylated), rabbit antibodies

AS09 547 | Anti-PPI | papain (affinity purified), rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans.

Alternative names: Papaya proteinase I, allergen= Car p 1.

Product citations

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