IKP

PPI | papain, Biotin conjugated

Product no: AS09 546

AS09 546 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

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  • Product Info
  • Immunogen: Native papain isolated and purified from Carica papaya
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Purified IgG in PBS.
    Format: Lyophilized
    Quantity: 10 mg
    Reconstitution: For reconstitution add 0,5 ml of sterile destilled water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
    Expected | apparent MW: 38,9 kDa
  • Reactivity
  • Confirmed reactivity: Carica papaya
    Predicted reactivity: Carica papaya
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
    Additional information (application): Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
  • Background
  • Background:

    Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans.

    Alternative names: Papaya proteinase I, allergen= Car p 1.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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