PPI | papain, Biotin conjugated
AS09 546 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya
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Product Information
Immunogen
native papain isolated and purified from Carica papaya
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG
Format
Lyophilized in phosphate buffer saline
Quantity
1 ml
Reconstitution
For reconstitution add 0.5 ml of sterile destilled water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
38.9 kDa
Reactivity
Confirmed reactivity
Carica papaya
Predicted reactivity
Carica papaya
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
Background
Background
Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans.
Alternative names: Papaya proteinase I, allergen= Car p 1.
Product citations
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