PPI | papain, Biotin conjugated

AS09 546 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

Product Information

Immunogen Native papain isolated and purified from Carica papaya

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 0,5 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW 38,9 kDa

Reactivity

Confirmed reactivity Carica papaya

Predicted reactivity Carica papaya

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Related products

AS09 545 | Anti-PPI | papain, rabbit antibodies

AS09 547 | Anti-PPI | papain (affinity purified), rabbit antibodies

Plant protein extraction buffer

Background

Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans.

Alternative names: Papaya proteinase I, allergen= Car p 1.

Product citations

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