PPI | Papain (affinity purified)
AS09 547 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

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Product Information
Immunogen
native papain isolated and purified from Carica papaya
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified IgG in PBS.
Format
Lyophilized
Quantity
1 mg
Reconstitution
For reconstitution add 0,5 ml of sterile destilled water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
38,9 kDa
Reactivity
Confirmed reactivity
Carica papaya
Predicted reactivity
Carica papaya
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran
Background
Background
Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans.
Alternative names: Papaya proteinase I, allergen= Car p 1.
Product citations
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