PPI | Papain (affinity purified)

AS09 547 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Carica papaya

Product Information

Immunogen native papain isolated and purified from Carica papaya

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified IgG in PBS.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 0,5 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Expected | apparent MW 38,9 kDa

Reactivity

Confirmed reactivity Carica papaya

Predicted reactivity Carica papaya

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran

Background

Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans.

Alternative names: Papaya proteinase I, allergen= Car p 1.

Product citations

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additional information:	Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran
background:	Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
Confirmed reactivity:	Carica papaya
predicted reactivity:	Carica papaya
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
calculated \| apparent molecular mass [kDa]:	38,9 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	native papain isolated and purified from Carica papaya
Purity:	Immunogen affinity purified IgG in PBS.
Quantity:	1 mg
recommended dilution:	1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 0,5 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)