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Pre-apoplastocyanin

AS06 118  | Clonality: Polyclonal  | Host: Rabbit  | Reactivity: Chlamydomonas reinhardtii

Pre-apoplastocyanin in the group Antibodies Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS06 118)
Pre-apoplastocyanin



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Product Information

Immunogen

GST fusion to Pre-apoplastocyanin of Chlamydomonas reinhartii  P18068

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17 kDa (precursor)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Chlorella fusca, Pediastrum boryanum
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information

This product can be sold containing ProClin if requested.

This antibody is recognizing pre-apoplatosyanin 17 kDa precursor and a mature protein. There is a slight cross-reactivity with cytochreom c6 in extract from Chlamydomonas reinhardtii cells grown under conditions of cooper deficiency.

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Background

Background

Plastocyanin is a “blue” copper protein which catalyzes electron transfer between the cytochrome b6 .f complex and P-700, the reaction center of photosystem I. Plastocyanin is a nuclear encoded polypeptide in all eukaryotic photosynthetic organisms where it has been studied. It is synthesized as a pre-protein of approximate molecular weight 17,000, imported post-translationally into chloroplasts, and processed to its mature form of approximate molecular weight 10,500 within the plastid.

Product citations

Selected references Li et al.  (1996).  Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.. J. Biol. Chem. 271:31283-31289
immunogen:

GST fusion to Pre-apoplastocyanin of Chlamydomonas reinhartii  P18068

Reconstitution: For reconstitution add 50 µl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 50 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]:

17 kDa (precursor)

Confirmed reactivity: Chlamydomonas reinhardtii
predicted reactivity: Chlorella fusca, Pediastrum boryanum
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information:

This product can be sold containing ProClin if requested.

additional information (application):

This antibody is recognizing pre-apoplatosyanin 17 kDa precursor and a mature protein. There is a slight cross-reactivity with cytochreom c6 in extract from Chlamydomonas reinhardtii cells grown under conditions of cooper deficiency.

background:

Plastocyanin is a “blue” copper protein which catalyzes electron transfer between the cytochrome b6 .f complex and P-700, the reaction center of photosystem I. Plastocyanin is a nuclear encoded polypeptide in all eukaryotic photosynthetic organisms where it has been studied. It is synthesized as a pre-protein of approximate molecular weight 17,000, imported post-translationally into chloroplasts, and processed to its mature form of approximate molecular weight 10,500 within the plastid.

All references: Li et al.  (1996).  Molecular genetic analysis of plastocyanin biosynthesis in Chlamydomonas reinhardtii.. J. Biol. Chem. 271:31283-31289

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