PsbX | small subunit X of PSII
AS05 066 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Data sheet | Product citations | Protocols | Add review |
Product Information
Immunogen
KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence UniProt: Q9SKI3
TAIR: At2g06520
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
200 ĩl
Reconstitution
For reconstitution add 200 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2500, 1 µg of chlorophyll/lane (WB)
Expected | apparent MW
4 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Dictos, Oryza sativa, Zea mays
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Cyanobacteria
Application examples
Additional information
Additional information
PsbX is a small (4 kDa) and very hydrophobic subunit of PSII, Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII, The use of 15% SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody
For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).
Background
Background
Plant PsbX is a nucelar encoded small 4 kDa protein associated with Photosystem II and found in all classes of oxygenic organisms.
Product citations
Selected references
Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.
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