PsbX | small subunit X of PSII

AS05 066 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence UniProt: Q9SKI3
TAIR: At2g06520

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 2500, 1 µg of chlorophyll/lane (WB)

Expected | apparent MW

4 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Dictos, Oryza sativa, Zea mays
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Not reactive in

Cyanobacteria

Application examples

Additional information

Additional information PsbX is a small (4 kDa) and very hydrophobic subunit of PSII, Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII, The use of 15% SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody

For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).

Background

Plant PsbX is a nucelar encoded small 4 kDa protein associated with Photosystem II and found in all classes of oxygenic organisms.

Product citations

Selected references Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.