PYK10 | Beta-Galactosidase (C-terminal)

Name: PYK10 | Beta-Galactosidase (C-terminal)
Brand: IKP
SKU: AS20 4410
Price: 323 EUR
Availability: InStock

AS20 4410 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PYK10 | Beta-Galactosidase (C-terminal) in the group Antibodies Plant/Algal / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS20 4410)

Product Information

Immunogen Conjugated peptide, derived from Arabidopsis thaliana C-terminal of PYK10, UniProt: A0A178VCN3, TAIR: At3g08880.

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format Liquid at 2 mg/ml.

Quantity 100 µg

Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1:500-1:1000 (IHC), 1: 5000- 1: 20 000 (WB)

Expected | apparent MW 59,7 | 56 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-PYK10 antibodies

Arabidopsis thaliana 7 day-old seedlings was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 12.5 % SDS-PAGE and blotted to PVDF membrane in semi-dry system. Blot was blocked with 5 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.

Additional information

N-terminal signal peptide including 24 amino acis and ER retention signal is removed from the mature protein

Background

Background PYK10 is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding. Cellular localisation: ER bodies.

Product citations

Selected references Matsushima et al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J. 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x.

immunogen:	Conjugated peptide, derived from Arabidopsis thaliana C-terminal of PYK10, UniProt: A0A178VCN3, TAIR: At3g08880.
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format:	Liquid at 2 mg/ml.
Quantity:	100 µg
storage:	Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1:500-1:1000 (IHC), 1: 5000- 1: 20 000 (WB)
Expected \| apparent MW:	59,7 \| 56 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Arabidopsis thaliana 7 day-old seedlings was freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. were separated on 12.5 % SDS-PAGE and blotted to PVDF membrane in semi-dry system. Blot was blocked with 5 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4000 in TBS-T for 1h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendations.
additional information (application):	N-terminal signal peptide including 24 amino acis and ER retention signal is removed from the mature protein
background:	PYK10 is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding. Cellular localisation: ER bodies.
All references:	Matsushima et al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J. 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x.

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