Rabbit anti-Goat IgG (H&L), ALP conjugated, min, cross-reactivity to human, mouse and Rat IgG

AS10 657 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Goat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Product Information

Immunogen

Purified Goat IgG, whole molecule

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified rabbit IgG.

Format Liquid

Quantity 0,5 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Goat IgG heavy and light chains (H&L)

Predicted reactivity Goat IgG Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to non-immunoglobulin goat serum proteins based or IgG from human, mouse or rat based on immunoelectrophoresis.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background

Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.

Product citations

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Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	Purified Goat IgG, whole molecule
Purity:	Immunogen affinity purified rabbit IgG.
Quantity:	0,5 mg
recommended dilution:	1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
additional information:	APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
additional information (application):	No reactivity is observed to non-immunoglobulin goat serum proteins based or IgG from human, mouse or rat based on immunoelectrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
background:	Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Confirmed reactivity:	Goat IgG heavy and light chains (H&L)
predicted reactivity:	Goat IgG Heavy and Light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known