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Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to human, mouse,Rat IgG

AS10 1058 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Goat IgG (H&L)

Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to human, mouse,Rat IgG  in the group Secondary Antibodies / Anti-Goat / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1058)



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Product Information

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified IgG, F(ab)'2 fragment.
Format Liquid
Quantity 0,5 mg
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC)

Reactivity

Application examples

Additional information

Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Related products

Background

Background

Rabbit anti-goat IgG (H&L) is a secondary antibody F(ab)'2 fragment, which binds to all goat IgGs in immunological assays and is FITC (fluorescein-5-isothiocyanate) conjugated. Antibody is also specially adsorbed against human,mouse and rat IgG.  FITC  Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE, may contain small amounts of intact IgG.

Product citations

Host: Rabbit
Clonality: Polyclonal
Purity: Immunogen affinity purified IgG, F(ab)'2 fragment.
Format: Liquid
Quantity: 0,5 mg
storage: Store at 2-8°C. Shelf life of this product is one year from date of receipt.
recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC)
additional information:

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

background:

Rabbit anti-goat IgG (H&L) is a secondary antibody F(ab)'2 fragment, which binds to all goat IgGs in immunological assays and is FITC (fluorescein-5-isothiocyanate) conjugated. Antibody is also specially adsorbed against human,mouse and rat IgG.  FITC  Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE, may contain small amounts of intact IgG.

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