Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to human, mouse,Rat IgG

Product no: AS10 1058

AS10 1058 | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Goat IgG (H&L)

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  • Product Info
  • Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgG, F(ab)'2 fragment.
    Format: Liquid
    Quantity: 0,5 mg
    Storage: Store at 2-8C. Shelf life of this product is one year from date of receipt.
    Recommended dilution: 1 : 50-1 : 5000 (ICC), 1 : 20-1 : 2000 (IHC)
  • Additional Information
  • Additional information:

    This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

    Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.

    Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

    BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

  • Background
  • Background:

    Rabbit anti-goat IgG (H&L) is a secondary antibody F(ab)'2 fragment, which binds to all goat IgGs in immunological assays and is FITC (fluorescein-5-isothiocyanate) conjugated. Antibody is also specially adsorbed against human,mouse and rat IgG.  FITC  Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE, may contain small amounts of intact IgG.

  • Protocols
  • Antibody protocols
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