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Rabbit anti-Goat IgG (H&L), FITC conjugated, min. cross-reactivity to human, mouse,Rat IgG

AS10 1271   | Clonality: Polyclonal  |  Host:Rabbit   |  Reactivity:Goat IgG (H&L)

Rabbit anti-Goat IgG (H&L), FITC conjugated, min. cross-reactivity to human, mouse,Rat IgG  in the group Secondary Antibodies / Anti-Goat / Fluorescent / FITC, TRITC at Agrisera AB (Antibodies for research) (AS10 1271)

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Product Information

Host Rabbit
Clonality Polyclonal
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg
Storage Store at 2-8°C.Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 50-1 : 5000 (ICC) (Flow cyt), 1 : 20-1 : 2000 (IHC)

Reactivity

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Additional information

Additional information

This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

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Background

Background Rabbit anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,mouse,rat IgG and affinity purified using solid phase goat IgG.

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