Rabbit anti-Goat IgG (H&L), HRP conjugated

Product no: AS09 605
AS09 605 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Goat IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Purified goat IgG, whole molecule
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified rabbit IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 10 000 -1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000 - 50 000 (WB)
  • Reactivity
  • Confirmed reactivity: Goat IgG heavy and light chains on all goat immunoglobulins
    Not reactive in: Non-immunoglobulin goat serum proteins based in immunoelectrophoresis
  • Application Examples
  • Application example

    western blot using secondary antibody rabbit anti-goat immunoglobulins, HRP conjugated

    5 µg of total extract from Arabidopsis thaliana leaf (S) extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary anti-BiP antibody (AS09 615) at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, AGRISERA, AS09 602) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent in extreme low femtogram range, according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

  • Additional Information
  • Additional information:

    Concentration: 1.0 mg/ml

    HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free with 0.1 % (v/v) of ProClin 150 as preservative.

    Additional information (application): For blocking BSA and non-fat milk is recommended to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.
  • Background
  • Background: Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.
  • Product Citations
  • Selected references: Sinclair et al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
  • Reviews:
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10 % until end of 2023. Use discount code: Conj10

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This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

AS16 ECL-S-1L, 1L

Choose the appropriate volume in the drop down menu to the right

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This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

AS16 ECL-N-1L, 1L

Choose the appropriate volume in the drop down menu to the right

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