BiP | Lumenal-binding protein (goat antibody)

AS09 615 | Clonality: Polyclonal | Host: Goat | Reactivity: Arabidopsis thaliana, Spinacia oleracea

Product Information

Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 UniProt:Q9LKR3, TAIR: At5g28540, BiP2 UniProt: F4K007, TAIR: At5g42020, BiP3 UniProt:Q8H1B3 ,TAIR:At1g09080

Host Goat

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

73.5 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays

Predicted reactivity Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Populus trichocarpa, Physcomitrella patens, Spinacia oleracea, Zea mays
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

western blot detection of plant BiP using goat antibodies

5 µg of total protein from A.thaliana (1), H. vulgare (2), Z.mays (3), S. oleracea (4), extracted with Agrisera PEB extraction buffer (AS08 300) were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-goat IgG horse radish peroxidase conjugated, from Agrisera AS09 605) diluted to 1:50 000 for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds.

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Antibody has a reduced reactivity to monocots in western blot.

Related products

AS09 614 | Anti-BiP2 | lumenal-binding protein, 2 chicken antibodies

AS09 481 | Anti-BiP2 | lumenal-binding protein 2, rabbit antibodies

antibodies to plant endomembrane system proteins

Plant protein extraction buffer

Secondary antibodies

Background

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2