showForm Anti-Anti-BiP | lumenal-binding protein hen antibodies

Name:
Phone:
E-mail:
Message

BiP | Lumenal-binding protein (chicken antibody)

AS09 614 | Clonality: Polyclonal | Host: Hen | Reactivity: Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays

BiP | Lumenal-binding protein (chicken antibody) in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS09 614)

DATA SHEET IN PDF

Qty: 
390
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 UniProt:Q9LKR3, TAIR: At5g28540, BiP2 UniProt: F4K007, TAIR: At5g42020, BiP3 UniProt:Q8H1B3 ,TAIR:At1g09080 

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY in PBS pH 7.4
Format Liquid
Quantity 100 µg
Storage Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Expected | apparent MW

73.5 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Physcomitrella patens, Spinacia oleracea, Zea mays
Predicted reactivity Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot detection of plant BiP using hen antibody

5 µg of total protein from  A.thaliana (1), H. vulgare (2) Z.mays (3) S. oleracea (4), extracted with Agrisera PEB extraction buffer (AS08 300) were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-hen IgY horse radish peroxidase conjugated, from  Agrisera AS09 603) diluted to 1:50 000  for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL  detection reagent according to the manufacturers instructions.  Exposure time was 5 seconds.


immunofluorescence

immunolocalization of plant BiP using hen antibody

BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-BiP primary antibody was diluted in 1: 1000 and DyLight®488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1: 1000. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

Additional information

Additional information

Antibody solution contains 0.02% sodium azide as preservative.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Related products

Related products

AS09 481 | Anti-BiP2 | lumenal-binding protein 2, rabbit antibodies

AS09 615 | Anti-BiP2 | lumenal-binding protein 2, goat antibodies

antibodies to plant endomembrane system proteins

Plant protein extraction buffer

Secondary antibodies

Background

Background

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

Product citations

Selected references Bennett et al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.

Related products: BiP | Lumenal-binding protein (chicken antibody)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 481 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, B.napus, Chara australis...
278 €
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items
AS09 622| Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)
190 €
AS09 603 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Hen IgY (H&L)
190 €