Rabbit anti-Rat IgG (H&L), TRITC conjugated

AS10 1525 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rat IgG (H&L)


product information
Background		Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Immunogen		Purified rat IgG (H&L)
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Affinity purified rabbit IgG
Format		Liquid
Quantity		1 mg
Reconstitution
Storage		Store at 2-8°C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Tested applications
Related products		other fluorescens conjugated secondary antibodies against rat IgG (H&L)
Additional information		Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth. Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.

application information
Recommended dilution		The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications.
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references

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