RAF2 | Rubisco accumulation factor 2
AS13 2729 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
|Info:||More information||Add review|
|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
18 | 17 kDa
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Arabidopsis alpina, Brassica napus, Capsella rubella, Glycine soja, Gpssypium aroboretum, Medicago trunculata, Morus notabilis, Ricinus communis, Theobroma cacao, Vitis vinifera|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Fristedt et al. (2018). RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. Plant J. 2018 Apr;94(1):146-156. doi: 10.1111/tpj.13849.
Aigner et al. (2017). Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2. Science. 2017 Dec 8;358(6368):1272-1278. doi: 10.1126/science.aap9221.
1-15µg of chlorophyll from isolated chloroplasts from Arabidopsis thaliana, extracted with a buffer containing (25 mM Tricine-NaOH, pH 7.8, 330 mM sorbitol, 1 mM EDTA, 10 mM KCl, 0.15% [w/v] bovine serum albumin, 4 mM sodium ascorbate, and 7 mM L-Cys) were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 10% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4⁰C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 60 seconds with a ImageQuant system from GE Healthcare, exposure time was 60 seconds.
Courtesy of Dr. Rikard Fristedt, University of Amsterdam, The Netherlands
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