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RAF2 | Rubisco accumulation factor 2

AS13 2729   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

RAF2 | Rubisco accumulation factor 2 in the group Plant/Algal Antibodies / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS13 2729)

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product information
Background

RAF2 (Rubisco accumulation factor 2) is a member if PCD family and a  chloroplastsic protein which cotains pterin carbinolamine dehydratase domain. Protein is involved in tetrahydrobiopterin biosynthetic process. Alternative names: AT5g51110/MWD22_5, PCD/DCoH-like protein 1, Transcriptional coactivator/pterin dehydrataseImported.

Immunogen

Recombinant, RAF2 protein UniProt:Q9LU63, TAIR: AT5G51110

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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matching Agrisera secondary antibody

collection of antibodies to chloroplastic proteins 

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

18 | 17 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis alpina, Brassica napus, Capsella rubella, Glycine soja, Gpssypium aroboretum, Medicago trunculata, Morus notabilis, Ricinus communis, Theobroma cacao, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Fristedt et al. (2018). RAF2 is a RuBisCO assembly factor in Arabidopsis thaliana. Plant J. 2018 Apr;94(1):146-156. doi: 10.1111/tpj.13849.
Aigner et al. (2017). Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2. Science. 2017 Dec 8;358(6368):1272-1278. doi: 10.1126/science.aap9221.

application example

western blot using anti-RAF2 antibodies

1-15µg of chlorophyll from isolated chloroplasts from Arabidopsis thaliana, extracted with a buffer containing (25 mM Tricine-NaOH, pH 7.8, 330 mM sorbitol, 1 mM EDTA, 10 mM KCl, 0.15% [w/v] bovine serum albumin, 4 mM sodium ascorbate, and 7 mM L-Cys) were separated on 12 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 10% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 overnight at 4⁰C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 60 seconds with a ImageQuant system from GE Healthcare, exposure time was 60 seconds.

Courtesy of Dr. Rikard Fristedt, University of Amsterdam, The Netherlands


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