RBP40 | 38 kDa RNA-binding protein
AS14 2820 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
42 | 40 kDa
Reactivity
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Chlamydomonas reinhardtii
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
application example

7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds.
Courtesy of Dr. Jörg Nickelsen, LMU München, Germany

7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds.
Courtesy of Dr. Jörg Nickelsen, LMU München, Germany
Additional information
Background
Background
RBP40 (38 kDa RNA-binding protein) binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo and specifically affects the initiation of D2 synthesis.
Alternative name: Chloroplast-targeted RNA-binding protein.
Product citations
Selected references
Schwarz at al.. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell, 19, 3627–3639.
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