RBP40 | 38 kDa RNA-binding protein
AS14 2820 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
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42 | 40 kDa
7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds.
Courtesy of Dr. Jörg Nickelsen, LMU München, Germany
RBP40 (38 kDa RNA-binding protein) binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo and specifically affects the initiation of D2 synthesis.
Alternative name: Chloroplast-targeted RNA-binding protein.