RBP40 | 38 kDa RNA-binding protein

Name: RBP40 | 38 kDa RNA-binding protein
SKU: AS14 2820
Price: 302 EUR
Availability: InStock

AS14 2820 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

RBP40 | 38 kDa RNA-binding protein in the group Antibodies Plant/Algal / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS14 2820)

Product Information

Immunogen

recombinant Chlamydomonas reinhardii RBR40 UniProt: Q6EMK7, AY124882

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

42 | 40 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example

western blot using anti-RBP40 / 38 kDa RNA-binding Protein antibodies

7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds.

Courtesy of Dr. Jörg Nickelsen, LMU München, Germany

Additional information

Background

RBP40 (38 kDa RNA-binding protein) binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo and specifically affects the initiation of D2 synthesis.

Alternative name: Chloroplast-targeted RNA-binding protein.

Product citations

Selected references Schwarz at al.. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell, 19, 3627–3639.

immunogen:	recombinant Chlamydomonas reinhardii RBR40 UniProt: Q6EMK7, AY124882
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunoprecipitation (IP), Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	42 \| 40 kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Chlamydomonas reinhardtii
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example 7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds. Courtesy of Dr. Jörg Nickelsen, LMU München, Germany
background:	RBP40 (38 kDa RNA-binding protein) binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo and specifically affects the initiation of D2 synthesis. Alternative name: Chloroplast-targeted RNA-binding protein.
All references:	Schwarz at al.. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell, 19, 3627–3639.