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Anti-RBP40 | 38 kDa RNA-binding protein
AS14 2820 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: recombinant Chlamydomonas reinhardii RBR40 UniProt: Q6EMK7, AY124882
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 42 | 40 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlamydomonas reinhardtii Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example 
7.5 µg of total protein from Chlamydomonas reinhardtii extracted with lysis buffer (120mM KCl, 20 mM Tricine pH 7.8, 0.4 mM EDTA, 5 mM ß-Mercaptoethanol and 1% Triton-X100), were separated on a 12 % SDS-PAGE and blotted 1h to nitrocellulose using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for ON at 4C with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Sigma, A9169) diluted to 1:10000 in 5% milk in TBS-T for 1h at RT with agitation. The blot was rinsed briefly once, then washed 4 times for 10 min and developed with ECL according to the manufacturer's instructions. Exposure time was 4 seconds.
Courtesy of Dr. Jörg Nickelsen, LMU München, Germany - Background
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Background: RBP40 (38 kDa RNA-binding protein) binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo and specifically affects the initiation of D2 synthesis.
Alternative name: Chloroplast-targeted RNA-binding protein. - Product Citations
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Selected references: Schwarz at al.. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell, 19, 3627–3639. - Reviews:
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