RDR2 | RNA-dependent RNA polymerase 2

Name: RDR2 | RNA-dependent RNA polymerase 2
SKU: AS15 3097
Price: 394 EUR
Availability: InStock

AS15 3097 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

RDR2 | RNA-dependent RNA polymerase 2 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 3097)

Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana RDR2 sequence, located towards C-terminal part of the protein, Uniprot: O82504, TAIR: AT4G11130

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB), coimmuno precipitation (coIP)

Recommended dilution 1 : 10 000 (WB)

Expected | apparent MW 129,3 | 130 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Arabidopsis lyrata

Not reactive in

Zea mays

Application examples

Application example

50 µg of total protein from Arabidopsis thaliana: rdr2-1 mutant, T-DNA insertion first exon (a), 30 µg protein from wild-type Col-0 (b), 50 µg protein from wild-type Col-0 (c), extracted with extraction buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with Laemmli buffer at 95°C/5 min., were separated on 7.5 % SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (5% milk powder; 1x TBS; 0.1% Tween-20) overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:10000 (a,b) and 1: 5000 (c) for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent and exposed to Amersham Hyperfilms ECL for 20 seconds.

Courtesy of Dr. Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina

Additional information

Background

RNA-dependent RNA polymerase 2 is a protein involved in the production of small interfering RNAs (siRNAs), transcriptional gene silencing (TGS), and it is a part of the RNA-directed DNA methylation (RdDM) silencing pathway.

Alternative name: RNA-directed RNA polymerase 2, Protein SILENCING MOVEMENT DEFICIENT 1.

Product citations

immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana RDR2 sequence, located towards C-terminal part of the protein, Uniprot: O82504, TAIR: AT4G11130
Reconstitution:	For reconstitution add 200 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	200 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB), coimmuno precipitation (coIP)
recommended dilution:	1 : 10 000 (WB)
calculated \| apparent molecular mass [kDa]:	129,3 \| 130 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Arabidopsis lyrata
not reactive in:	Zea mays
Picture (footer):	Application example 50 µg of total protein from Arabidopsis thaliana: rdr2-1 mutant, T-DNA insertion first exon (a), 30 µg protein from wild-type Col-0 (b), 50 µg protein from wild-type Col-0 (c), extracted with extraction buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with Laemmli buffer at 95°C/5 min., were separated on 7.5 % SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (5% milk powder; 1x TBS; 0.1% Tween-20) overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:10000 (a,b) and 1: 5000 (c) for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent and exposed to Amersham Hyperfilms ECL for 20 seconds. Courtesy of Dr. Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina
background:	RNA-dependent RNA polymerase 2 is a protein involved in the production of small interfering RNAs (siRNAs), transcriptional gene silencing (TGS), and it is a part of the RNA-directed DNA methylation (RdDM) silencing pathway. Alternative name: RNA-directed RNA polymerase 2, Protein SILENCING MOVEMENT DEFICIENT 1.

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