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RDR2 | RNA-dependent RNA polymerase 2

AS15 3097 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

RDR2 | RNA-dependent RNA polymerase 2 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 3097)

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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana RDR2 sequence, located towards C-terminal part of the protein, Uniprot: O82504, TAIR: AT4G11130
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB), coimmuno precipitation (coIP)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

129.3 | 130 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis lyrata
Not reactive in

Zea mays

Application examples

Application examples

Application example


western blot using anti-RDR2 antibodies

50 µg of total protein from Arabidopsis thaliana: rdr2-1 mutant, T-DNA insertion first exon (a), 30 µg protein from wild-type Col-0 (b), 50 µg protein from wild-type Col-0 (c),  extracted with extraction buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with Laemmli buffer at 95°C/5 min., were separated on 7.5 % SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (5% milk powder; 1x TBS; 0.1% Tween-20) overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:10000 (a,b) and 1: 5000 (c) for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent and exposed to Amersham Hyperfilms ECL for 20 seconds.

Courtesy of Dr. Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina

Additional information

Related products

Related products

AS15 3096 | Anti-RDR1 | RNA-dependent RNA polymerase 1, rabbit antibodies
AS15 3098 | Anti-RDR6 | RNA-dependent RNA polymerase 6, rabbit antibodies

Collection of antibodies to proteins involved in regulation of transcription

Plant protein extraction buffer

Secondary antibodies

Background

Background

RNA-dependent RNA polymerase 2 is a protein involved in the production of small interfering RNAs (siRNAs), transcriptional gene silencing (TGS), and it is a part of the RNA-directed DNA methylation (RdDM) silencing pathway.

Alternative name: RNA-directed RNA polymerase 2, Protein SILENCING MOVEMENT DEFICIENT 1.

Product citations

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