RDR6 | RNA-dependent RNA polymerase 6

Product no: AS15 3098

AS15 3098 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

302
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana RDR6 sequence, Uniprot: Q9SG02 ,TAIR: At3g49500
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000-1 : 6000 (WB)
    Expected | apparent MW: 136,9 | 130 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in:

    Nicotiana tabacum, Solanum lycopersicum, Zea mays

  • Application Examples
  • Application example

    Western blot using anti-RDR6 antibodies

    50 µg of total protein from Arabidopsis thaliana whole vegetative rosette wild type Col-0 (a) rdr6-12 mutant (b) extracted with extraction buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with Laemmli buffer at 95°C/5 min., were separated on 7.5 % SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (5% milk powder; 1x TBS; 0.1% Tween-20) overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:6000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent and expose to Amersham Hyperfilms ECL for 3 minutes. 

    Courtesy of Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina

  • Background
  • Background:

    RDR6 (RNA-dependent RNA polymerase 6) is involved in trans-acting siRNA and other siRNA biogenesis and required for post-transcriptional gene silencing and natural virus resistance.

    Alternative names:Protein silencing defective 1, Protein supressor of gene silencing 2, RNA.directed RNSA polymerase 6.

  • Protocols


  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
Add to cart
Info

AS15 3097 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

394 €
Add to cart
Info
Close