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RDR6 | RNA-dependent RNA polymerase 6

AS15 3098 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

RDR6 | RNA-dependent RNA polymerase 6 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 3098)

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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana RDR6 sequence, Uniprot: Q9SG02 ,TAIR: At3g49500
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000-1 : 6000 (WB)
Expected | apparent MW 136.9 | 130 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in

Nicotiana tabacum, Solanum lycopersicum, Zea mays

Application examples

Application examples Application example

Western blot using anti-RDR6 antibodies

50 µg of total protein from Arabidopsis thaliana whole vegetative rosette wild type Col-0 (a) rdr6-12 mutant (b) extracted with extraction buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM EDTA; 10 % v/v Glycerin; 1 mM DTT, 1x Complete Protease Inhibitor Cocktail, Roche) and denatured with Laemmli buffer at 95°C/5 min., were separated on 7.5 % SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were blocked with blocking buffer (5% milk powder; 1x TBS; 0.1% Tween-20) overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:6000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly and then washed tree times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:20 000 in blocking buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent and expose to Amersham Hyperfilms ECL for 3 minutes. 

Courtesy of Dr. Pablo Manavella, Instituto de Agrobiotecnología del Litoral (IAL), Argentina

Additional information

Related products

Related products AS15 3096 | Anti-RDR1 | RNA-dependent RNA polymerase 1, rabbit antibodies

AS15 3097 | Anti-RDR2 | RNA-dependent RNA polymerase 2, rabbit antibodies

Antibodies to proteins involved in regulation of transcription

Plant protein extraction buffer

Secondary antibodies

Background

Background

RDR6 (RNA-dependent RNA polymerase 6) is involved in trans-acting siRNA and other siRNA biogenesis and required for post-transcriptional gene silencing and natural virus resistance.

Alternative names:Protein silencing defective 1, Protein supressor of gene silencing 2, RNA.directed RNSA polymerase 6.

Product citations

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