Ricin (RTA subunit), clone MMA
AS09 648 | Clonality: Polyclonal | Host: Mouse | Reactivity: Ricinus communis
|Data sheet||Product citations||Protocols||Add review|
Ricin, RTA subunit
Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1%, dextran 1%, NaCl 100mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine.
The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/ml) or by Bradford method
Ricin detection limit is 5 ng/ml, signal-to-noise ratio is 15-50 up to 100 ng/ml
Epitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule.
Suggested protocol can be found here.
Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.