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Ricin (whole molecule), clone MMH

AS09 649 | Clonality: Polyclonal | Host: Mouse | Reactivity: Ricinus communis

Ricin (whole molecule), clone MMH in the group Antibodies for Plant/Algal  / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 649)

DATA SHEET IN PDF

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385
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

Ricin, whole molecule

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified IgG
Format Lyophilized in phosphate buffer saline pH 7.4, contains mannitol
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store at -70°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. For storage at 4°C sodium azide can be added.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC)
Recommended dilution The optimal working dilution should be determined by the investigator. for specific application
Expected | apparent MW

29.8 kDa

Reactivity

Confirmed reactivity Ricinus communis
Predicted reactivity Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant.

Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml

As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.

Related products

Related products

AS09 648 | Anti-Ricin (RTA subunit), clone MMA, mouse antibodies

AS17 4143 | Anti-RTA | Ricin toxic A chain, rabbit polyclonal antibodies
AS17 4144 | Anti-RTB | Ricin toxic B chain, rabbit polyclonal antibodies

collection of antibodies to food proteins

Secondary antibodies

Background

Background

Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell. 

Product citations

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