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Ricin (whole molecule), clone MMH

AS09 649 | Clonality: Polyclonal | Host: Mouse | Reactivity: Ricinus communis

Ricin (whole molecule), clone MMH in the group Antibodies Plant/Algal  / Toxins / Plant toxins at Agrisera AB (Antibodies for research) (AS09 649)



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Product Information

Immunogen

Ricin, whole molecule

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified IgG in PBS pH 7.4 with mannitol.
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store at -70°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added
Tested applications ELISA (ELISA), Immunohistochemistry (IHC)
Recommended dilution The optimal working dilution should be determined by the investigator, for specific application
Expected | apparent MW 29,8 kDa

Reactivity

Confirmed reactivity Ricinus communis
Predicted reactivity Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information

Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant.

Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml

As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.

Related products

Background

Background

Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell. 

Product citations

calculated | apparent molecular mass [kDa]: 29,8 kDa
Clonality: Monoclonal
Format: Lyophilized
Host: Mouse
immunogen:

Ricin, whole molecule

Purity: Purified IgG in PBS pH 7.4 with mannitol.
Quantity: 200 ĩg
recommended dilution: The optimal working dilution should be determined by the investigator, for specific application
Reconstitution: For reconstitution add 200 ĩl of sterile water
storage: Store at -70°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added
Sub class: IgG1
tested applications: ELISA (ELISA), Immunohistochemistry (IHC)
background:

Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell. 

Confirmed reactivity: Ricinus communis
predicted reactivity: Ricinus communis
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information:

Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant.

Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml

As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.

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