IKP

Ricin (whole molecule), clone MMH

Product no: AS09 649

AS09 649 | Clonality: Polyclonal | Host: Mouse | Reactivity: Ricinus communis

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  • Product Info
  • Sub class: IgG1
    Immunogen:

    Ricin, whole molecule

    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified IgG in PBS pH 7.4 with mannitol.
    Format: Lyophilized
    Quantity: 200 g
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store at -70C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4C sodium azide can be added
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC)
    Recommended dilution: The optimal working dilution should be determined by the investigator, for specific application
    Expected | apparent MW: 29,8 kDa
  • Reactivity
  • Confirmed reactivity: Ricinus communis
    Predicted reactivity: Ricinus communis
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant.

    Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml

    As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.

  • Background
  • Background:

    Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell. 

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • SANDWICH ELISA FOR DETERMINATION OF RICIN


    Materials and Reagents
    1. 96-well plates
    2. Suitable plate reader (depending on the detection system)
    3. Plate shaker with temperature control
    4. Capture antibody (#177 YO Proteins)
    5. Detection antibody (conjugate) (enquire)
    6. Washing solution (PBS, 0.025% Tween 20, 1% D-lactose)
    7. Solution for blocking and dilution (PBS, 0.025% Tween 20, 1% D-lactose,
    0.1% BSA)
    8. TMB reagent
    9. Sulfuric acid 10%

    Protocol
    1. Immobilisation of capture antibody
    Dissolve capture antibody in PBS at 5 μg/ml. Add 100 μl into each well. Incubate 2
    hours at 37°C. Wash twice with 300 μl washing solution.
    2. Blocking
    Add 250 ml blocking solution. Incubate 1 hour at 37°C. Wash with washing solution
    twice 300 μl.
    3. Application of control and samples.
    Add 100 μl to each well: antigen (ricin) diluted from 0.5 to 100 ng/ml, sample serially
    diluted. Incubate 1 hour at 37°C. Washing 3 times 300 μl.
    4. Detection antibody (example based on biotin conjugate)
    Add 100 μl of biotin-conjugated antibody (1 μg/ml). Incubate 1 hour at 37°C.
    Washing 3 times 300 μl (not shorter than 5 min each).
    5. Streptavidin-HRP conjugate
    Add 100 μl of Streptavidin-HRP conjugate (diluted 10000X). Incubate 1 hour at
    37°C. Washing 5 times 300 μl (not shorter than 5 min each).
    6. Development
    Add 100 μl of development TMB solution. Incubate 10 min at 37°C. Stop reaction
    with 10 μl 10% sulfuric acid. Read OD at 450 nm

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