Ricin (whole molecule), clone MMH

AS09 649 | Clonality: Polyclonal | Host: Mouse | Reactivity: Ricinus communis

Product Information

Immunogen

Ricin, whole molecule

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Purified IgG in PBS pH 7.4 with mannitol.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store at -70°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added

Tested applications ELISA (ELISA), Immunohistochemistry (IHC)

Recommended dilution The optimal working dilution should be determined by the investigator, for specific application

Expected | apparent MW 29,8 kDa

Reactivity

Confirmed reactivity Ricinus communis

Predicted reactivity Ricinus communis

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant.

Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml

As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.

Background

Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.

Product citations

Related products: Ricin (whole molecule), clone MMH

Rabbit anti-Mouse IgG (H&L), HRP conjugated

AS09 627 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L...

206 €

Ricin (RTA subunit), clone MMA

AS09 648 | Clonality: Polyclonal | Host: Mouse | Reactivity: Ricinus communis

407 €

RTA | Ricin toxic A chain

AS17 4143 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ricinus com...

302 €

Buy 2 items of this product for 226.00 €/items

Buy 3 items of this product for 205.00 €/items

RTB | Ricin toxic B chain

AS17 4144 | Clonality: Polyclonal | Host: Rabbit | Reactivity: ...

302 €

Buy 2 items of this product for 226.00 €/items

Buy 3 items of this product for 205.00 €/items

calculated \| apparent molecular mass [kDa]:	29,8 kDa
Clonality:	Monoclonal
Format:	Lyophilized
Host:	Mouse
immunogen:	Ricin, whole molecule
Purity:	Purified IgG in PBS pH 7.4 with mannitol.
Quantity:	200 µg
recommended dilution:	The optimal working dilution should be determined by the investigator, for specific application
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store at -70°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added
Sub class:	IgG1
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC)
background:	Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.
Confirmed reactivity:	Ricinus communis
predicted reactivity:	Ricinus communis
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant. Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.