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RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

AS19 4265  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

RPN5a | 26S proteasome regulatory subunit, putative (RPN5) in the group Antibodies for Plant/Algal  / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4265)

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Product Information

Immunogen Recombinant RPN5a of Arabidopsis thaliana UniProt:F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW 52.7 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycine soja, Gossypium arboreum, Juglans regia, Morus notabilis, Noccaea caerulescens, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is also recognizing RPN5b.

Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio. 

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AS19 4262 | Anti-RPT2a | Regulatory particle triple-A ATPase subunit 2a, rabbit antibodies
AS19 4263 | Anti-RPT4a | 26S proteasome AAA-ATPase subunit RPT4a, rabbit antibodies

AS19 4264  | Anti-26S proteasome regulatory subunit RPN1a , rabbit antibodies

collection of antibodies to protein modifications and enzymes involved in this process

Background

Background RPN5a | 26S proteasome regulatory subunit, putative (RPN5) is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.

Product citations

Selected references Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

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