RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

Product no: AS19 4265

AS19 4265  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: Recombinant RPN5a of Arabidopsis thaliana UniProt:F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C (short tem, months) or at -80C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: 52,7 | 50 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Glycine soja, Gossypium arboreum, Juglans regia, Morus notabilis, Noccaea caerulescens, Theobroma cacao
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): This antibody is also recognizing RPN5b.

    Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio. 
  • Background
  • Background: RPN5a | 26S proteasome regulatory subunit, putative (RPN5) is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.
  • Product Citations
  • Selected references: Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
  • Reviews:
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    Number of reviews: (1)
    Jun Lee | 2023-07-28
    0 | 0
    -15 ug of total protein from Arabidopsis thaliana leaves
    -Primary antibody at a dilution of 1:1000 o/n at 4C
    -Secondary antibody (AS09 602) at 1:50000 dilution for 1hr at RT
    -Detect HRP using SuperSignal West Pico PLUS

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AS19 4264  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS19 4266  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

302 €
Add to cart
Info
Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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