RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

Name: RPN5a | 26S proteasome regulatory subunit, putative (RPN5)
SKU: AS19 4265
Price: 302 EUR
Availability: InStock

AS19 4265 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

RPN5a | 26S proteasome regulatory subunit, putative (RPN5) in the group Antibodies Plant/Algal / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4265)

RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

Product Information

Immunogen Recombinant RPN5a of Arabidopsis thaliana UniProt:F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW 52,7 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Glycine soja, Gossypium arboreum, Juglans regia, Morus notabilis, Noccaea caerulescens, Theobroma cacao
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is also recognizing RPN5b.

Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.

Background

Background RPN5a | 26S proteasome regulatory subunit, putative (RPN5) is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.

Product citations

Selected references Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

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immunogen:	Recombinant RPN5a of Arabidopsis thaliana UniProt:F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece
Reconstitution:	For reconstitution add 50 µl, of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 5000 (WB)
calculated \| apparent molecular mass [kDa]:	52,7 \| 50 kDa
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Glycine soja, Gossypium arboreum, Juglans regia, Morus notabilis, Noccaea caerulescens, Theobroma cacao Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	This antibody is also recognizing RPN5b. Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.
background:	RPN5a \| 26S proteasome regulatory subunit, putative (RPN5) is one of two isoforms for the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions it acts redundantly with the paralogous gene RPN5b but also appears to exert independent effects.
All references:	Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.