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RPN10 | 26S proteasome regulatory subunit RPN10

AS19 4266  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

RPN10 | 26S proteasome regulatory subunit RPN10 in the group Antibodies for Plant/Algal  / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4266)

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Product Information

Immunogen HIS-tagged RPN10 of Arabidopsis thaliana UniProt: P55034-1, TAIR: At4g38630 expressed in E. coli and purified by metal-chelate affinity chromatography
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 40.7 | 40 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Gossypium hirsutum, Noccaea caerulescens, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

For western blot detection image, please check van Nocker et al. (1996).

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AS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodies
AS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodies

AS19 4262 | Anti-RPT2a | Regulatory particle triple-A ATPase subunit 2a, rabbit antibodies
AS19 4263 | Anti-RPT4a | 26S proteasome AAA-ATPase subunit RPT4a, rabbit antibodies

AS19 4264  | Anti-26S proteasome regulatory subunit RPN1a , rabbit antibodies
AS19 4265 | Anti-RPN5a | 26S proteasome regulatory subunit, putative (RPN5), rabbit antibodies

collection of antibodies to protein modifications and enzymes involved in this process

Background

Background RPN10 (26S proteasome regulatory subunit RPN10) is playing a role in maintaining the structural integrity of the 19S regulatory particle (RP), subcomplex of the 26S proteasome by the direct and indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP). Binds and presumably selects ubiquitin-conjugates for destruction. Plays a role in the growth and development via the proteasome-dependent degradation of the ABA-signaling protein ABI5/DPBF1 and in the balancing cell expansion with cell proliferation rates during shoot development.

Alternative names: 26S proteasome non-ATPase regulatory subunit 4 homolog, AtRPN10, 26S proteasome regulatory subunit S5A homolog, Multiubiquitin chain-binding protein 1, AtMCB1, MBP1.

Product citations

Selected references van Nocker et al. (1996). Arabidopsis MBP1 gene encodes a conserved ubiquitin recognition component of the 26S proteasome. Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):856-60.

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