RPN10 | 26S proteasome regulatory subunit RPN10

AS19 4266  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

RPN10 | 26S proteasome regulatory subunit RPN10 in the group Antibodies Plant/Algal  / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4266)
RPN10 | 26S proteasome regulatory subunit RPN10


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Product Information

Immunogen HIS-tagged RPN10 of Arabidopsis thaliana UniProt: P55034-1, TAIR: At4g38630 expressed in E. coli and purified by metal-chelate affinity chromatography


Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl, of sterile water
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 40,7 | 40 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Gossypium hirsutum, Noccaea caerulescens, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

For western blot detection image, please check van Nocker et al. (1996).

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collection of antibodies to protein modifications and enzymes involved in this process


Background RPN10 (26S proteasome regulatory subunit RPN10) is playing a role in maintaining the structural integrity of the 19S regulatory particle (RP), subcomplex of the 26S proteasome by the direct and indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP). Binds and presumably selects ubiquitin-conjugates for destruction. Plays a role in the growth and development via the proteasome-dependent degradation of the ABA-signaling protein ABI5/DPBF1 and in the balancing cell expansion with cell proliferation rates during shoot development.

Alternative names: 26S proteasome non-ATPase regulatory subunit 4 homolog, AtRPN10, 26S proteasome regulatory subunit S5A homolog, Multiubiquitin chain-binding protein 1, AtMCB1, MBP1.

Product citations

Selected references Huang et al (2021). Parasitic modulation of host development by ubiquitin-independent protein degradation. Cell. 2021 Sep 30;184(20):5201-5214.e12. doi: 10.1016/j.cell.2021.08.029. Epub 2021 Sep 17. PMID: 34536345; PMCID: PMC8525514.
van Nocker et al. (1996). Arabidopsis MBP1 gene encodes a conserved ubiquitin recognition component of the 26S proteasome. Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):856-60.

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