RPN1a 26S proteasome regulatory subunit RPN1a

AS19 4264 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen Recombinant, full length Arabidopsis thaliana RPN1a protein sequence UniProt: Q9SIV2-1, TAIR: At2g20580 overexpressed in E.coli, purified from a gel piece

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water.

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 98.1 | 100 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Actinidia chinensis var. chinensis, Artemisia annua, Brassica napus, Brassica oleracea var. oleracea, Cajanus cajan, Capsella rubella, Capsicum baccatum, Cephalotus follicularis, Cicer arietinum, Citrus clementina, Corchorus capsularis, Cucumis melo, Cucumis sativus, Eucalyptus grandis, Eutrema salsugineum, Fagus sylvatica, Glycine max, Gossypium raimondii, Helianthus annuus, Jatropha curcas, Lactuca sativa, Lupinus angustifolius, Macleaya cordata, Medicago truncatula, Mucuna pruriens, Nelumbo nucifera, Nicotiana attenuata, Nicotiana sylvestris, Nicotiana tabacum, Noccaea caerulescens, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Punica granatum, Ricinus communis, Rosa chinensis, Solanum tuberosum, Theobroma cacao, Trifolium pratense, Vitis vinifera
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Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Background

Background RPN1a (26S proteasome regulatory subunit RPN1a) Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins (By similarity). Required during embryogenesis and involved in innate immune response, proteasome-mediated ubiquitin-dependent protein catabolic process, protein catabolic process, regulation of cell cycle, regulation of protein catabolic process, response to salicylic acid, ubiquitin-dependent protein catabolic process.
Alternative names: 26S proteasome non-ATPase regulatory subunit 2 homolog A, AtRPN1a, 26S proteasome regulatory subunit S2 homolog A.

Product citations

Selected references Yang et al. (2004). Purification of the Arabidopsis 26 S proteasome: biochemical and molecular analyses revealed the presence of multiple isoforms. J Biol Chem. 2004 Feb 20;279(8):6401-13.