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RpoA | RNA polymerase alpha subunit (chloroplast)

AS15 2866 | clonality: polyclonal | host: rabbit | reactiviy: Arabidopsis thaliana

RpoA | RNA polymerase alpha subunit (chloroplast)  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 2866)

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Product Information

Immunogen His-tagged, highly conserved fragment of Zea mays RpoA UniProt: P09562
Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein G
Format Lyophilized
Quantity 2 mg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1: 500 (WB)
Expected | apparent MW 38 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Zea mays
Species of your interest not listed? Contact us
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Application examples

Application examples Western blot using anti-RpoA antibodies

Stromal fraction proteins (100 µg) were extracted from Arabidopsis thaliana cell culture grown under dark (7-d-D) and 150 μmol m-2s -1 constant white light for 7 days (7-d-L). Proteins were separated by NativePAGE 3-12% Bis-Tris Gel and detected using anti-RpoB antibody. PEP complex separated by BN-PAGE were further resolved by 4-12% NuPAGE and blotted 250mA for 1.5h to 0.45 µm PVDF using wet transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody anti-RpoA at a dilution of 1: 500 for ON/4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly three times, then washed once for 10 min and repeat 3 times in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated; AS09 602) diluted to 1:10 000 for 1h/RT with agitation. The blot was washed as above and developed with AgriseraECL SuperBright (AS16 ECL-S) for 12 sec for dark sample and 2 sec for light sample.

Courtesy Yan Ji, PhD student Åsa Strand Group Department of Plant Physiology Umeå Plant Science Centre (UPSC), Sweden

Additional information

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Background

Background RpoA | RNA polymerase alpha subunit (chloroplast) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Product citations

Selected references Ji et al. (2020).  A fully assembled PEP complex detected in etioplasts and proplastids in Arabidopsis. Physiol Plant. 2020 Nov 5. doi: 10.1111/ppl.13256.

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