RpoB | RNA polymerase beta subunit (chloroplast)
AS15 2867 | Clonality: Polyclonal | Host: Rabbit | Reactiviy: Arabidopsis thaliana
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Product Information
Immunogen
His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
100 ĩg
Reconstitution
For reconstitution add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500 (WB)
Expected | apparent MW
121 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Alloteropsis semialata, Cannabis sativa, Coleataenia prionitis, Digitaria exilis, Echinochloa crus-galli var. crus-galli , Eragrostis tef, Microlaena stipoides, Miscanthus sacchariflorus, Oryza sativa, Phragmites australis, Potamophila parviflora, Rhynchoryza subulata, Saccharum officinarum, Setaria italica, Sorghum bicolor, Stipa lipskyi, Sporobolus michauxianus, Triticum aestivum
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
Stroma, thylakoid and total protein from Arabidopsis thaliana were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent reagent of extreme low femtogram range. Exposure time was 120 seconds.
Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA
Stroma, thylakoid and total protein from Arabidopsis thaliana were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent reagent of extreme low femtogram range. Exposure time was 120 seconds.
Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA
Additional information
Antibody does not work on total cell extracts. Stromal fraction has to be used.
This antibody is detecting recombinant RpoB.
This antibody is detecting recombinant RpoB.
Background
Background
RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.
Product citations
Selected references
Zhang et al. (2017). PDM3, a pentatricopeptide repeat-containing protein, affects chloroplast development. J Exp Bot. 2017 Nov 28;68(20):5615-5627. doi: 10.1093/jxb/erx360.
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