RpoC1 | RNA polymerase beta' subunit (chloroplast)
AS15 2868 | Clonality: Polyclonal | Host: Rabbit | Reactiviy: Arabidopsis thaliana, Zea mays
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Product Information
Immunogen
His-tagged, highly conserved fragment of Zea mays RpoC.
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
100 ĩg
Reconstitution
For reconstitution, add 50 ĩl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500-1 : 1000 (WB)
Expected | apparent MW
78 | 78 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Zea mays
Predicted reactivity
Alloteropsis semialata, Aristida purpurea, Chasmanthium latifolium, Chaetobromus involucratus subsp. dregeanus, Chionochloa macra, Coleataenia prionitis, Danthonia californica , Digitaria exilis, Echinochloa oryzicola, Eragrostis tef, Eremitis sp., Isachne distichophylla, Hilaria cenchroides , Hickelia madagascariensis, Microlaena stipoides, Miscanthus sinensis, Neololeba atra, Olmeca reflexa, Oryza sativa, Otatea acuminata, Panicum miliaceum , Pariana campestris, Phragmites australis, Potamophila parviflora, Puelia olyriformis, Rhynchoryza subulata, Saccharum hybrid, Saccharum officinarum, Setaria viridis, Sorghum bicolor, Sorghum timorense, Sporobolus maritimus, Stipa purpurea, Triticum aestivum, Zoysia macrantha
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
Stroma, thylakoid, nuclei and total protein from Arabidopsis thaliana and Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent detection reagent of extreme low femtogram range. Exposure time was 120 seconds.
Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA
Stroma, thylakoid, nuclei and total protein from Arabidopsis thaliana and Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent detection reagent of extreme low femtogram range. Exposure time was 120 seconds.
Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA
Additional information
There may be occur a cross reacting band at 100 kDa in maize samples
Background
Background
RpoC1 (RNA polymerase beta' subunit (chloroplast)) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Product citations
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