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RpoC1 | RNA polymerase beta' subunit (chloroplast)

AS15 2868 | Clonality: Polyclonal | Host: Rabbit | Reactiviy: Arabidopsis thaliana, Zea mays

RpoC1 | RNA polymerase beta' subunit (chloroplast)  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 2868)

DATA SHEET IN PDF

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286
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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen His-tagged, highly conserved fragment of Zea mays RpoC.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution, add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 500-1 : 1000 (WB)
Expected | apparent MW

78 | 78 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Alloteropsis semialata, Aristida purpurea, Chasmanthium latifolium, Chaetobromus involucratus subsp. dregeanus, Chionochloa macra, Coleataenia prionitis, Danthonia californica , Digitaria exilis, Echinochloa oryzicola, Eragrostis tef, Eremitis sp., Isachne distichophylla, Hilaria cenchroides , Hickelia madagascariensis, Microlaena stipoides, Miscanthus sinensis, Neololeba atra, Olmeca reflexa, Oryza sativa, Otatea acuminata, Panicum miliaceum , Pariana campestris, Phragmites australis, Potamophila parviflora, Puelia olyriformis, Rhynchoryza subulata, Saccharum hybrid, Saccharum officinarum, Setaria viridis, Sorghum bicolor, Sorghum timorense, Sporobolus maritimus, Stipa purpurea, Triticum aestivum, Zoysia macrantha
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-RpoC antibodies

Stroma, thylakoid, nuclei and total protein from Arabidopsis thaliana and Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent detection reagent of extreme low femtogram range. Exposure time was 120 seconds.

Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA

Additional information

There may be occur a cross reacting band at 100 kDa in maize samples.

Related products

Related products

AS15 2866 | Anti-RpoA | RNA polymerase alpha subunit (chloroplast), rabbit antibodies
AS15 2867A | Anti-RpoB | RNA polymerase beta subunit (chloroplast) for maize, rabbit antibodies
AS15 2867 | Anti-RpoB | RNA polymerase beta subunit (chloroplast) for Arabidopsis thaliana, rabbit antibodies

collection of antibodies to proteins involved in transcription

Background

Background RpoC1 (RNA polymerase beta' subunit (chloroplast))  is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Product citations

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