RpoC1 | RNA polymerase beta' subunit (chloroplast)

Product no: AS15 2868

AS15 2868 | Clonality: Polyclonal | Host: Rabbit | Reactiviy: Arabidopsis thaliana, Zea mays

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  • Product Info
  • Immunogen: His-tagged, highly conserved fragment of Zea mays RpoC.
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: For reconstitution, add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 500-1 : 1000 (WB)
    Expected | apparent MW:

    78 | 78 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Zea mays
    Predicted reactivity: Alloteropsis semialata, Aristida purpurea, Chasmanthium latifolium, Chaetobromus involucratus subsp. dregeanus, Chionochloa macra, Coleataenia prionitis, Danthonia californica , Digitaria exilis, Echinochloa oryzicola, Eragrostis tef, Eremitis sp., Isachne distichophylla, Hilaria cenchroides , Hickelia madagascariensis, Microlaena stipoides, Miscanthus sinensis, Neololeba atra, Olmeca reflexa, Oryza sativa, Otatea acuminata, Panicum miliaceum , Pariana campestris, Phragmites australis, Potamophila parviflora, Puelia olyriformis, Rhynchoryza subulata, Saccharum hybrid, Saccharum officinarum, Setaria viridis, Sorghum bicolor, Sorghum timorense, Sporobolus maritimus, Stipa purpurea, Triticum aestivum, Zoysia macrantha
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-RpoC antibodies

    Samples:

    Left panel: Zea mays extracts: whole leaf (5 ug), chloroplast nucleoid (0.1 ug), thylakoid (5 ug), or stroma (5 ug) as indicated MW markers are marked

    5 µg/well of total protein extracted from Arabidopsis thaliana and Zea mays in 100 mM Tris-HCL pH7.5, 10%glycerol, 5mM EDTA and 2MM EGTA buffer and denatured in 66mM Tris-HCL pH6.8, 0.2% glycerol, 0.23% SDS, 600mM BME at 50°C/10 min. Samples were separated on 4-20 % SDS-PAGE Novex™ Tris-Glycine Mini Protein Gels (4–20%, 1.0 mm thick) and blotted overnight to nitrocellulose (0.22um), using: wet transfer. Blot was blocked with 5 % milk for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:500 for 2h/RT with agitation in 5% non-fat milk TBS-T with agitation. The antibody solution was decanted, and the blot was rinsed briefly 4 times in TBS-T for 5min each with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:1000 in 5% non-fat milk TBS-T for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent AgriseraECL SuperBright. Exposure time was 5 minutes.

    Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA



    western blot using anti-RpoC antibodies

    Stroma, thylakoid, nuclei and total protein from Arabidopsis thaliana and Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent detection reagent of extreme low femtogram range. Exposure time was 120 seconds.

    Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA

  • Additional Information
  • Additional information (application): There may be occur a cross reacting band at 100 kDa in maize samples
  • Background
  • Background: RpoC1 (RNA polymerase beta' subunit (chloroplast))  is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
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    Number of reviews: (2)
    Wojciech Majeran | 2024-03-01
    0 | 0
    Used with Zea mays, protein load 5 ug total, clean signal on chloroplast and nucleoid fractions. Dilution 1:2000 works well in nucleoid enriched fractions, total chloroplast requires 1:1000 dilution
    Reference yu Qing-bo, ph.d | 2019-04-25
    0 | 0
    It works well against Arabidopsis thylakoid protein.

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