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RpoB | RNA polymerase beta subunit (chloroplast) (maize)

AS15 2867A | Clonality: Polyclonal | Host: Rabbit | Reactiviy: Zea mays

RpoB | RNA polymerase beta subunit (chloroplast) (maize) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS15 2867A)

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Product Information

Immunogen His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 500 (WB)
Expected | apparent MW

121 kDa

Reactivity

Confirmed reactivity Zea mays
Predicted reactivity Alloteropsis semialata, Coleataenia prionitis, Digitaria exilis, Echinochloa crus-galli var. crus-galli , Eragrostis tef, Microlaena stipoides, Hordeum vulgare, Miscanthus sacchariflorus, Oryza sativa, Phragmites australis, Potamophila parviflora, Rhynchoryza subulata, Saccharum officinarum, Setaria italica, Sorghum bicolor, Stipa lipskyi, Sporobolus michauxianus, Triticum aestivum
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example


western blot using anti-RpoB

Stroma, thylakoid, nuclei and total protein from Zea mays were extracted with 50 mM HEPES-KOH pH 8, 330 mM sorbitol exaction buffer. Components were separated on 12% SDS-PAGE and blotted overnight to Nitrocellulose membrane using wet transfer. Blots were blocked with 5% non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5min with chemiluminescent detection reagent of extreme low femtogram range. Exposure time was 120 seconds.

Courtesy of Jie Shen and Dr. Alice Barkan, University of Oregon, USA

Additional information

Antibody does not work on total cell extracts. Stromal fraction has to be used.

This antibody is detecting recombinant RpoB.

Related products

Related products AS15 2866 | Anti-RpoA | RNA polymerase alpha subunit (chloroplast), rabbit antibodies
AS15 2867 | Anti-RpoB | RNA polymerase beta subunit (chloroplast), rabbit antibodies

AS15 2868 | Anti-RpoC1 | RNA polymerase beta' subunit (chloroplast) , rabbit antibodies

collection of antibodies to proteins involved in transcription


Background

Background RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.

Product citations

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