RPS12 | Ribosomal protein S12 (chloroplastic)

Product no: AS12 2114

AS12 2114 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii

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  • Product Info
  • Immunogen:

    Recombinant full length RPS12 of Chlamydomonas reinhardtii, P14149, expressed in E.coli

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW: 14,6 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays
    Predicted reactivity:
    Bacteria, Dunaliella salina, Cannabis sativa, Chlorella vulgaris, Nannochloropsis gaditana, Oltmannsiellopsis viridis (marine flagellate), Pinus sp., Physcomitrium patens, Prochlorococcus marinus, Scenedesmus obliquus, Spinacia oleracea, Synechocystis sp. PCC6803, Synechococcus elongatus PCC 7942, Volvox carteri (green alga)

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

    western blot using anti-RPS12 antibodies

    10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors)  were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk  for 1h at RT with agitation. The antibody solution was decanted and the blot was washed  3 times for 15 min  in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:0 000 in TBS-T containing 1% non-fat dry milk  for 1h at RT with agitation. The blot was washed as above  and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.
     

    Courtesy of Dr. Silvia Ramundo, University of Geneva, Switzerland

     

     

     

  • Additional Information
  • Additional information: This product can be sold containing proclin if requested
  • Background
  • Background:

    RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

  • Product Citations
  • Selected references: Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .
    Zoschke et al. (2016). The PPR-SMR protein PPR53 enhances the stability and translation of specific chloroplast RNAs in maize. Plant J. 2016 Mar;85(5):594-606. doi: 10.1111/tpj.13093. Epub 2016 Feb 5.
    Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.
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