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RPS15 | 30S ribosomal protein S15, chloroplastic

AS14 2779 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana,Oryza sativa, Zea mays

RPS15 | 30S ribosomal protein S15, chloroplastic in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS14 2779)
RPS15 | 30S ribosomal protein S15, chloroplastic



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Product Information

Immunogen

KLH-conjugated peptide, derived from Arabidopsis thaliana RPS15 UniProt: P56805, TAIR: AtCg01120

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution for reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution

1: 1000

Expected | apparent MW

10 kDa

Reactivity

Confirmed reactivity Nicotiana tabacum
Predicted reactivity Arabidopsis thaliana, Solanum lycopersicum, Solanum tuburosum, Oryza sativa, Zea mays

Species of your interest not listed? Contact us
Not reactive in

 

Application examples

Application examples Western blot using anti-RPS15 antibodies

5 µg/well of total protein extracted freshly from Nicotiana tabacum RPS15 mutant (1) and wildtype (2) with 0.7 M sucrose, 0.5 M Tris, 50 mM EDTA, 0.1 M KCl, 2% β-Mercaptoethanol, 2% Complete proteases inhibitor, and Phenol, and denatured with 1 vol of 2x protein loading buffer (125 mM Tris-HCl (pH 6.8), 4% SDS, 20% glycerol, 25 mM EDTA, 0.4 mg/ml Bromophenol blue, 2% β-Mercaptoethanol) at 64°C for 10 min. Samples were separated on 12 % SDS-PAGE and blotted 20V/ON or 350A/2h at 4°C to nitrocellulose (pore size of 0.2 µm), using wet transfer. Blot was blocked with 4% milk for 30min/RT on a gentle rocking. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for ON/4°C on a gentle rocking in TBS-T. The antibody solution was decanted, and the blot was rinsed briefly with TBS-T, then washed three times for 10min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat Anti-Rabbit IgG (H + L)-HRP Conjugated) diluted to 1:10 000 in 2% dry milk, 15 mM EDTA and TBS-T for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent following manufacture's recommendations. Exposure time was 1.5 min.


Courtesy of Drs Jinghan Liu and Reimo Zoschke, Max Planck Institute of Molecular Plant Physiology, Germany

Additional information

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Background

Background RPS15 (30S ribosomal protein S15, chloroplastic) is a structural component of the small subunit of the ribosome, localized in the chloroplast.

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