RPS15 | 30S ribosomal protein S15, chloroplastic

Product no: AS14 2779

AS14 2779 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana,Oryza sativa, Zea mays

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  • Product Info
  • Immunogen:

    KLH-conjugated peptide, derived from Arabidopsis thaliana RPS15 UniProt: P56805, TAIR: AtCg01120

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: for reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution:

    1: 1000

    Expected | apparent MW:

    10 kDa

  • Reactivity
  • Confirmed reactivity: Nicotiana tabacum
    Predicted reactivity: Arabidopsis thaliana, Solanum lycopersicum, Solanum tuburosum, Oryza sativa, Zea mays

    Species of your interest not listed? Contact us
    Not reactive in:

     

  • Application Examples
  • Western blot using anti-RPS15 antibodies

    5 µg/well of total protein extracted freshly from Nicotiana tabacum RPS15 mutant (1) and wildtype (2) with 0.7 M sucrose, 0.5 M Tris, 50 mM EDTA, 0.1 M KCl, 2% β-Mercaptoethanol, 2% Complete proteases inhibitor, and Phenol, and denatured with 1 vol of 2x protein loading buffer (125 mM Tris-HCl (pH 6.8), 4% SDS, 20% glycerol, 25 mM EDTA, 0.4 mg/ml Bromophenol blue, 2% β-Mercaptoethanol) at 64°C for 10 min. Samples were separated on 12 % SDS-PAGE and blotted 20V/ON or 350A/2h at 4°C to nitrocellulose (pore size of 0.2 µm), using wet transfer. Blot was blocked with 4% milk for 30min/RT on a gentle rocking. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for ON/4°C on a gentle rocking in TBS-T. The antibody solution was decanted, and the blot was rinsed briefly with TBS-T, then washed three times for 10min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (Goat Anti-Rabbit IgG (H + L)-HRP Conjugated) diluted to 1:10 000 in 2% dry milk, 15 mM EDTA and TBS-T for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent following manufacture's recommendations. Exposure time was 1.5 min.


    Courtesy of Drs Jinghan Liu and Reimo Zoschke, Max Planck Institute of Molecular Plant Physiology, Germany

  • Background
  • Background: RPS15 (30S ribosomal protein S15, chloroplastic) is a structural component of the small subunit of the ribosome, localized in the chloroplast.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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