RPS14 | Ribosomal protein S14 (mitochondrial)
AS16 4094 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana RPS14 protein sequence. UniProt: O64695 TAIR:
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum in PBS, pH 7.4
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
15.6 | 16 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Amborella trichopoda, Anthurium amnicola, Brassica juncea, Brassica oleracea var. botrytis, Capsella rubella, Carica papaya, Castilleja paramensis, Cycas taitungensis, Eruca sativa, Erythranthe guttata, Eschscholzia californica, Flagellaria indica, Glycine max, Gossypium raimondii, Guzmania lingulata, Heuchera parviflora var. saurensis, Hesperelaea palmeri, Liriodendron tulipifera, Luzula sylvatica, Medicago truncatula, Nelumbo nucifera, Nicotiana tabacum, Nymphaea tetragona, Oenothera berteroana, Pisum sativum, Phaseolus angularis , Phoenix dactylifera, Poa saltuensis subsp. languida, Populus canescens, Raphanus sativus, Silene latifolia, Sinapis arvensis, Typha latifolia, Vicia faba, Vigna radiata, Vitis vinifera
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Application examples
Application examples
Application example
30 µg of mitochondrial fraction from Arabidopsis thaliana extracted with buffer (sucrose, tetrasodiumpyrophosphate, EDTA, KH2O4, PVP-40, BSA, pH 7.5) and denatured with 56°C for 15 min at 650 rpm were separated on 12 % SDS-PAGE and blotted overnight (12V) to PVDF using wet transfer. Blots were blocked with 5% skimmed powder milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the purified anti S14 serum at a dilution of 1: 1 000 for 1,5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent. Exposure time was ~ 60 seconds.
Courtesy of Dr. Małgorzata Kwaśniak-Owczarek, University of Wrocław, Poland
30 µg of mitochondrial fraction from Arabidopsis thaliana extracted with buffer (sucrose, tetrasodiumpyrophosphate, EDTA, KH2O4, PVP-40, BSA, pH 7.5) and denatured with 56°C for 15 min at 650 rpm were separated on 12 % SDS-PAGE and blotted overnight (12V) to PVDF using wet transfer. Blots were blocked with 5% skimmed powder milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the purified anti S14 serum at a dilution of 1: 1 000 for 1,5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent. Exposure time was ~ 60 seconds.
Courtesy of Dr. Małgorzata Kwaśniak-Owczarek, University of Wrocław, Poland
Additional information
Background
Background
RPS14 | Ribosomal protein S14 (mitochondrial) is a structural constituent of ribosome and is localized in mitochondria.
Product citations
Selected references
Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .
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