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RPT2a | Regulatory particle triple-A ATPase subunit 2a

AS19 4262   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

RPT2a  | Regulatory particle triple-A ATPase subunit 2a in the group Antibodies for Plant/Algal  / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4262)

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Product Information

Immunogen Recombinant, full lenght RPT2a of Arabidopsis thaliana RPT2a protein sequence UniProt: Q9SZD4-1, TAIR: At4g29040 overexpressed in E.coli, purified from a gel piece
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 49.4 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Brassica napus, Cajanus cajan, Gossypium hirsutum, Mucuna pruriens, Noccaea caerulescens, Vigna radiata var. radiata
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is also recognizing RPT2b protein. 

Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.

For an image of western blot detection, refer to: Smalle et al. (2002).

Related products

Related products AS19 4260 | Anti-PBA1 | 20S Proteasome beta subunit A1, rabbit antibodies
AS19 4258 | PAC1 | 20S Proteasome alpha subunit C1
AS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodies
AS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodies

collection of antibodies to protein modifications and enzymes involved in this process

Background

Background RPT2a (Regulatory particle triple-A ATPase subunit 2a) is the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation.

Alternative names: 26S proteasome regulatory subunit 4 homolog A Alternative name(s): 26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, Protein HALTED ROOT, 26S proteasome regulatory subunit 4 homolog A.

Product citations

Selected references Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

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