RPT2a | Regulatory particle triple-A ATPase subunit 2a

Name: RPT2a | Regulatory particle triple-A ATPase subunit 2a
SKU: AS19 4262
Price: 302 EUR
Availability: InStock

AS19 4262 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

RPT2a | Regulatory particle triple-A ATPase subunit 2a in the group Antibodies Plant/Algal / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4262)

RPT2a | Regulatory particle triple-A ATPase subunit 2a

Product Information

Immunogen Recombinant, full lenght RPT2a of Arabidopsis thaliana RPT2a protein sequence UniProt: Q9SZD4-1, TAIR: At4g29040 overexpressed in E.coli, purified from a gel piece

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 49,4 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Brassica napus, Cajanus cajan, Gossypium hirsutum, Mucuna pruriens, Noccaea caerulescens, Vigna radiata var. radiata
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is also recognizing RPT2b protein.

Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.

For an image of western blot detection, refer to: Smalle et al. (2002).

Background

Background RPT2a (Regulatory particle triple-A ATPase subunit 2a) is the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation.

Alternative names: 26S proteasome regulatory subunit 4 homolog A Alternative name(s): 26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, Protein HALTED ROOT, 26S proteasome regulatory subunit 4 homolog A.

Product citations

Selected references Meng, Wang, Hao, et al. (2023) RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis. Plant Physiol. 2023;191(1):446-462. doi:10.1093/plphys/kiac511
Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

background:	RPT2a (Regulatory particle triple-A ATPase subunit 2a) is the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation. Alternative names: 26S proteasome regulatory subunit 4 homolog A Alternative name(s): 26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, Protein HALTED ROOT, 26S proteasome regulatory subunit 4 homolog A.
calculated \| apparent molecular mass [kDa]:	49,4 \| 50 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Recombinant, full lenght RPT2a of Arabidopsis thaliana RPT2a protein sequence UniProt: Q9SZD4-1, TAIR: At4g29040 overexpressed in E.coli, purified from a gel piece
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 3000 (WB)
Reconstitution:	For reconstitution add 50 µl, of sterile water
storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
additional information (application):	This antibody is also recognizing RPT2b protein. Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA. For an image of western blot detection, refer to: Smalle et al. (2002).
All references:	Meng, Wang, Hao, et al. (2023) RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis. Plant Physiol. 2023;191(1):446-462. doi:10.1093/plphys/kiac511 Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
Confirmed reactivity:	Arabidopsis thaliana
predicted reactivity:	Brassica napus, Cajanus cajan, Gossypium hirsutum, Mucuna pruriens, Noccaea caerulescens, Vigna radiata var. radiata Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known