RPT4a | 26S proteasome AAA-ATPase subunit RPT4a

AS19 4263 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

Product Information

Immunogen Full length recombinant RPT4a of Arabidopsis thaliana, UniProt: Q9SEI3-1, TAIR: At5g43010, overexpressed in E.coli

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water.

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 44.8 | 44 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays

Predicted reactivity Actinidia chinensis, Artemisia annua, Capsicum chinense, Cicer arietinum, Helianthus annuus, Medicago truncatula, Nicotiana sylvestris, Noccaea tabacum, Solanum tuberosum, Trifolium pratense, Ricinus communis, Vigna radiata
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

This antibody is also recognizing RPT4b isofrom.

Related products

Background

Background RPT4a (26S proteasome AAA-ATPase subunit RPT4a) is involved in positive regulation of RNA polymerase II transcriptional preinitiation complex assembly, ubiquitin-dependent ERAD pathway, ubiquitin-dependent protein catabolic process.

Alternative names: 26S proteasome subunit 10B homolog A, Regulatory particle triple-A ATPase subunit 4a, 26S proteasome regulatory subunit 10B homolog A.

Product citations

Selected references Marshall et al. (2015). Autophagic Degradation of the 26S Proteasome Is Mediated by the Dual ATG8/Ubiquitin Receptor RPN10 in Arabidopsis. Mol Cell. 2015 Jun 18;58(6):1053-66. doi: 10.1016/j.molcel.2015.04.023.