PAC1 | 20S Proteasome alpha subunit C1

Name: PAC1 | 20S Proteasome alpha subunit C1
SKU: AS19 4258
Price: 302 EUR
Availability: InStock

AS19 4258 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PAC1 | 20S Proteasome alpha subunit C1 in the group Antibodies Plant/Algal / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4258)

Product Information

Immunogen Recombinant PAC1 of Arabidopsis thaliana, UniProt: O81148-1, TAIR: At3g22110 , overexpressed in E.coli, purified from a gel piece

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 27,4 | 26 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.

For an image of western blot detection, refer to: Smalle et al. (2002).

Background

Background PAC1 (20S Proteasome alpha subunit C1) is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent.

Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3

Product citations

Selected references Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

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immunogen:	Recombinant PAC1 of Arabidopsis thaliana, UniProt: O81148-1, TAIR: At3g22110 , overexpressed in E.coli, purified from a gel piece
Reconstitution:	For reconstitution add 50 µl, of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 3000 (WB)
calculated \| apparent molecular mass [kDa]:	27,4 \| 26 kDa
Confirmed reactivity:	Arabidopsis thaliana
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA. For an image of western blot detection, refer to: Smalle et al. (2002).
background:	PAC1 (20S Proteasome alpha subunit C1) is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent. Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3
All references:	Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.