Name:
Phone:
E-mail:
Address:


 


PAC1 | 20S Proteasome alpha subunit C1

AS19 4258   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

PAC1 | 20S Proteasome alpha subunit C1 in the group Antibodies for Plant/Algal  / Protein Modifications / Plant Proteasome at Agrisera AB (Antibodies for research) (AS19 4258)

DATA SHEET IN PDF

Qty: 
292
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant PAC1 of Arabidopsis thaliana, UniProt: O81148-1, TAIR: At3g22110 , overexpressed in E.coli, purified from a gel piece
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 (WB)
Expected | apparent MW 27.4 | 26 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.

For an image of western blot detection, refer to: Smalle et al. (2002).

Related products

Related products AS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodies
AS19 4260 | Anti-PBA1 | 20S Proteasome beta subunit A1, rabbit antibodies
AS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodies

collection of antibodies to protein modifications and enzymes involved in this process

Background

Background PAC1 (20S Proteasome alpha subunit C1) is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent. 

Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3

Product citations

Selected references Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

Related products: PAC1 | 20S Proteasome alpha subunit C1

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €
AS19 4259   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsi...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS19 4260   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsi...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items