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Anti-PAC1 | 20S Proteasome alpha subunit C1
Product no: AS19 4258
AS19 4258 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
329
€
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- Product Info
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Immunogen: Recombinant PAC1 of Arabidopsis thaliana, UniProt: O81148-1, TAIR: At3g22110, overexpressed in E.coli, purified from a gel piece Host: Rabbit
Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 3000 (WB) Expected | apparent MW: 27.4 | 26 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.
For an image of western blot detection, refer to: Smalle et al. (2002). - Background
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Background: PAC1 (20S Proteasome alpha subunit C1) is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent.
Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3 - Product Citations
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Selected references: Pang et al. (2025). The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis. Nat Commun. 2025 Feb 27;16(1):2039. doi: 10.1038/s41467-025-57306-7.
Xie et al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.
Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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