PBA1 | 20S proteasome beta subunit A1

AS19 4260 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

Product Information

Immunogen Full length, soluble PBA1 of Arabidopsis thaliana, UniProt: F4JRY2-1, TAIR: At4g31300, overexpressed in E.coli

Host

Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW 25,3 | 24 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Zea mays

Predicted reactivity Actinidia chinensis var. chinensis, Amborella trichopoda, Ananas comosus, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Capsicum baccatum, Cephalotus follicularis, Citrus sinensis, Coffea canephora, Corchorus olitorius, Cuscuta australis, Daucus carota subsp. sativus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Fagus sylvatica, Gossypium hirsutum, Handroanthus impetiginosus, Jatropha curcas, Juglans regia, Manihot esculenta, Nelumbo nucifera, Nicotiana attenuata, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Prunus persica, Phoenix dactylifera, Rhizophora mucronata, Rosa chinensis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Solanum tuberosum, Theobroma cacao, Trema orientale, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Background

Background PBA1 (20S proteasome beta subunit A1) is a subunit of proteasome, multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.

Alternative name: Proteasome subunit beta.

Product citations

Selected references Boussardon, Bag, Juvany, et al. (2022) The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence. Commun Biol. 2022;5(1):1043. Published 2022 Sep 30. doi:10.1038/s42003-022-03998-2
Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

calculated \| apparent molecular mass [kDa]:	25,3 \| 24 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	Full length, soluble PBA1 of Arabidopsis thaliana, UniProt: F4JRY2-1, TAIR: At4g31300, overexpressed in E.coli
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 3000 (WB)
Reconstitution:	For reconstitution add 50 µl, of sterile water
storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
background:	PBA1 (20S proteasome beta subunit A1) is a subunit of proteasome, multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Alternative name: Proteasome subunit beta.
All references:	Boussardon, Bag, Juvany, et al. (2022) The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence. Commun Biol. 2022;5(1):1043. Published 2022 Sep 30. doi:10.1038/s42003-022-03998-2 Smalle et al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
Confirmed reactivity:	Arabidopsis thaliana, Zea mays
predicted reactivity:	Actinidia chinensis var. chinensis, Amborella trichopoda, Ananas comosus, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Capsicum baccatum, Cephalotus follicularis, Citrus sinensis, Coffea canephora, Corchorus olitorius, Cuscuta australis, Daucus carota subsp. sativus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Fagus sylvatica, Gossypium hirsutum, Handroanthus impetiginosus, Jatropha curcas, Juglans regia, Manihot esculenta, Nelumbo nucifera, Nicotiana attenuata, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Prunus persica, Phoenix dactylifera, Rhizophora mucronata, Rosa chinensis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Solanum tuberosum, Theobroma cacao, Trema orientale, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known